IFO50014 MDBK (NBL-1) Cell information
Cell type：general cells
(View Pricing Information )
JCRB No.
IFO50014
Cell Name
MDBK (NBL-1)
Profile
established from normal kidney
Other Name
Animal
bovine
Strain
Genus
Bos
Species
taurus
Sex
Age
adult
Identity
Tissue for Primary Cancer
kidney
Case history
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
established from normal kidney
Classify
normal
Established by
Madin, S. H. & Darby, N. B.
Registered by
Takeuchi,M.
Regulation for Distribution
Comment
Year
1985
Medium
Eagle's minimal essential medium with 10% fetal bovine serum
Methods for Passages
trypsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
kidney
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Images
LOT Information
Cell No.
IFO50014
Cell Name
MDBK (NBL-1)
LOT No.
78
Lot Specification
distribution
Medium
E.MEM, 90% ; FBS, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
3 x 10^6
Viability at cell freezing (%)
Antibiotics Used
free
Passage Number
Unknown (6 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, G6PD, MD, AST
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Labeled as ”IFO50014, R27F78”. No cells name is appeared in the label but it is the correct vial.
