IFO50019 NIH 3T3 p-7 cl-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50019 Cell Name   NIH 3T3 p-7 cl-3
Profile   fibroblast (NIH Swiss mouse) Other Name   
Animal   mouse Strain   NIH Swiss
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblasts from embryonic mouse.
Classify   normal Established by   Hakura, A.
Registered by   Hakura,A. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_L994
Reference
Pubmed id:4087463Two NIH3T3 cell lines of different origins circulating in the world.
Iwamoto A,Masuda M,Yoshikura H
Jpn J Exp Med. 1985 Jun;55(3):129-31
Pubmed id:3918269Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells.
Mulcahy LS,Smith MR,Stacey DW
Nature. 1985 Jan 17-23;313(5999):241-3
Pubmed id:742018[Therapeutic results in adult acute leukemia in Giessen].
Löffler H,Pralle H,Graubner M,Schrezenmaier E
Verh Dtsch Ges Inn Med. 1978;(84):156-8
Pubmed id:4311790Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
Jainchill JL,Aaronson SA,Todaro GJ
J Virol. 1969 Nov;4(5):549-53

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LOT Information

Cell No.   IFO50019 Cell Name   
LOT No.   01252011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 3.6 x 10^4 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Do not allow the cells to full confluency.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50019 Cell Name   NIH 3T3 p-7 cl-3
LOT No.   344 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (>3 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50019 Cell Name   NIH 3T3 p-7 cl-3
LOT No.   340 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (>3 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,MD,AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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