IFO50021 A31-714 C4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50021 Cell Name   A31-714 C4
Profile   fibroblast, subclone of Balb/3T3 A31, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/c
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast, subclone of Balb/3T3 A31. This cell line exhibits contact inhibition.
Classify   normal Established by   Vogt, P.
Registered by   Hakura,A. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_L989
Reference
Pubmed id:4792350A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3.
Kakunaga T
Int J Cancer. 1973 Sep 15;12(2):463-73

Images



LOT Information

Cell No.   IFO50021 Cell Name   A31-714 C4
LOT No.   339 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   1-3 x 10^5/5 ml/60mm dish Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^5
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50021 Cell Name   A31-714 C4
LOT No.   341 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   1-3 x 10^5/5 ml/60mm dish Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4 x 10^5
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, MPI, PepB, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
CLOSE