IFO50039 NC-37
Cell information
Important Notice(s)IFO50039 NC-37 is derivative from Raji.
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50039 | Cell Name | NC-37 |
|---|---|---|---|
| Profile | lymphoblast, Contamination of Raji cells has been suspected. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 34 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | lymphoblast, Contamination of Raji cells has been suspected. |
| Classify | tumor | Established by | Durr, F. E. et al. |
| Registered by | Takeuchi,M. | Regulation for Distribution | |
| Comment | Contamination of Raji cells has been suspected in this line. This cell line contains EB virus genom. | Year | 1985 |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:4321017 | Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Durr FE,Monroe JH,Schmitter R,Traul KA,Hirshaut Y Int J Cancer. 1970 Nov 15;6(3):436-49 |
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LOT Information
| Cell No. | IFO50039 | Cell Name | NC-37 |
|---|---|---|---|
| LOT No. | 424 | Lot Specification | distribution |
| Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | dilution | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3 x 10^6 | |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | Unknown (7 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), MD, PepB |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |