IFO50069 Alexander cells
Cell information
Important Notice(s)The Alexander cells: another name of PLC/PRF/5 cell line.
Cell type:general cells (View Pricing Information)
JCRB No. | IFO50069 | Cell Name | Alexander cells |
---|---|---|---|
Profile | hepatoma, HBs antigen-positive. This cell line is the same cell line as PLF/PRF/5 (other name of PLC/PRF/5). | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | M | Age | 24 |
Identity | available | Tissue for Primary Cancer | liver, gallbladder |
Case history | malignant liver cancer | Metastasis | No |
Tissue Metastasized | Genetics | ||
Life Span | infinite | Crisis PDL | |
Morphology | epithelial-like | Character | hepatoma, HBs antigen-positive |
Classify | tumor | Established by | Alexander, J. J. |
Registered by | Takeuchi,M. | Regulation for Distribution | |
Comment | Year | 1985 | |
Medium | RPMI1640 medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
Cell Number on Passage | Race | ||
CO2 Conc. | 5 % | Tissue Sampling | liver |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
---|---|---|---|---|---|---|---|---|---|
Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | + | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
---|---|
Pubmed id:63998 | Establishment of a continuously growing cell line from primary carcinoma of the liver. Alexander JJ,Bey EM,Geddes EW,Lecatsas G S Afr Med J. 1976 Dec 18;50(54):2124-8 |
Pubmed id:187208 | Hepatitis B surface antigen produced by a human hepatoma cell line. MacNab GM,Alexander JJ,Lecatsas G,Bey EM,Urbanowicz JM Br J Cancer. 1976 Nov;34(5):509-15 |
Images |
---|
LOT Information
Cell No. | IFO50069 | Cell Name | Alexander cells |
---|---|---|---|
LOT No. | 1011 | Lot Specification | distribution |
Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
Doubling Time | Cell Number in Vial (cells/1ml) | 6 x 10^5 | |
Viability at cell freezing (%) | 93 | Antibiotics Used | free |
Passage Number | Unknown (6 at IFO) | PDL | |
Sterility: MYCOPLASMA | + | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type A), MD |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Exoteric Gene | ||
Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50069 | Cell Name | Alexander cells |
---|---|---|---|
LOT No. | 10252013 | Lot Specification | distribution |
Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). | Temperature | 37C |
Cell Density at Seeding | 3.5 - 4.4 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin and 0.02% EDTA. |
Doubling Time | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 (cells/1 | |
Viability at cell freezing (%) | Antibiotics Used | free | |
Passage Number | Unknown (11 at bank) | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeA),NP,LDH were examined. Human |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:11,12 D7S820:9,11 D16S539:13 VWA:15,16 TH01:7,8 AM:X TPOX:8 CSF1PO:10 |
|
Adhesion | Exoteric Gene | ||
Medium for Freezing | CO2 Conc. | 5% | |
Viability immediately after thawing (%) | 89.0 | Additional information |