IFO50071 MDCK (NBL-2)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50071 Cell Name   MDCK (NBL-2)
Profile   Epitherial-like cell line established from normal kidney of dog, susceptible for many viral species. Other Name   MDCK
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex   F Age   adult
Identity    Tissue for Primary Cancer   kidney
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   established from normal kidney, susceptible for many viral species
Classify   normal Established by   Madin, S. H. & Darby, N. B.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0422
Reference
Pubmed id:15234806Comparative study of influenza virus replication in Vero and MDCK cell lines.
Youil R,Su Q,Toner TJ,Szymkowiak C,Kwan WS,Rubin B,Petrukhin L,Kiseleva I,Shaw AR,DiStefano D
J Virol Methods. 2004 Sep 1;120(1):23-31
Pubmed id:212055A cell strain cultured from porcine kidney increases cyclic AMP content upon exposure to calcitonin or vasopressin.
Goldring SR,Dayer JM,Ausiello DA,Krane SM
Biochem Biophys Res Commun. 1978 Jul 28;83(2):434-40
Pubmed id:567227Polarized monolayers formed by epithelial cells on a permeable and translucent support.
Cereijido M,Robbins ES,Dolan WJ,Rotunno CA,Sabatini DD
J Cell Biol. 1978 Jun;77(3):853-80
Pubmed id:828141The origin and characteristics of a pig kidney cell strain, LLC-PK.
Hull RN,Cherry WR,Weaver GW
In Vitro. 1976 Oct;12(10):670-7
Pubmed id:4248968A cell line derived from normal dog kidney (MDCK) exhibiting qualities of papillary adenocarcinoma and of renal tubular epithelium.
Leighton J,Estes LW,Mansukhani S,Brada Z
Cancer. 1970 Nov;26(5):1022-8
Pubmed id:13567776Established kidney cell lines of normal adult bovine and ovine origin.
MADIN SH,DARBY NB Jr
Proc Soc Exp Biol Med. 1958 Jul;98(3):574-6

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LOT Information

Cell No.   IFO50071 Cell Name   
LOT No.   05242004 Lot Specification   distribution
Medium   EMEM + 10% FBS(FBS lot; GIBCO 3762782S) Temperature   37C
Cell Density at Seeding   8 x 10^4cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   3 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as dog by NP, G6PD, AST, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   YES Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS, 70% EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50071 Cell Name   MDCK (NBL-2)
LOT No.   190 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   Unknown PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, MPI, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50071 Cell Name   MDCK (NBL-2)
LOT No.   11242016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   8.7 - 9.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA for 15 min.
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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