-Detailed Information [IFO50271]-

IFO50271 HUV-EC-C

Cell information

Important Notice(s)

[IFO50271 HUV-EC-Cのウイルス検査成績について]

本細胞株におきましては、ヒト・ヘルペスウイルス6のウイルスゲノムが検出されますが、ホストゲノムに挿入されたウイルスゲノムコピー数は1コピーであり、継代とともに変化することがないため、ウイルスの複製は起こっていないものとみられます。

また、一般的にウイルスの産生を誘導するとされる薬剤(sodium butylate,TPA)による処理によってもコピー数は変化しませんでした。このことから、ヘルペスウイルスゲノムの挿入はみられるものの、ウイルスの産生は起こっていないと判断しています。

(文献)
Cytotechnology (2017). https://doi.org/10.1007/s10616-017-0119-y 
PubMed ID: 28755030

(*1) The HHV6B genome fragment was detected. However, the number of copy of integration of HHV6B to host genome was one and unchanged during passages, showing that HHV6B is not produced by HUV-EC-C. In addition, HHV6 viral replication was not observed even by the stimulation with chemicals.

Reference
Cytotechnology (2017). https://doi.org/10.1007/s10616-017-0119-y 
PubMed ID: 28755030

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Cell type：general cells (View Pricing Information)

JCRB No.	IFO50271	Cell Name	HUV-EC-C
Profile	Normal endothelial cells with limited life span. For cultivation, endothelial cell growth supplement can be substituted by ca 10 ng/ml basic FGF.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	vascular system
Case history		Metastasis
Tissue Metastasized		Genetics
Life Span	finite	Crisis PDL	50-60
Morphology	endothelial	Character	normal endothelial cell, umbilical cord
Classify	primary	Established by	Hoshi, H.
Registered by	Suzuki,N.	Regulation for Distribution
Comment		Year	1990
Medium	MCDB 107 with 10% FBS, 100ug/ml heparin and 50ug/ml endothelial cell growth supplement	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	umbilical cord, vein
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	+	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes	The HSV6B genome fragment was detected by realtime PCR. However, the copy number of HSV6B integrated to host genome was one and unchanged during passages. In addition, HSV6 viral replication was not observed even by the stimulation with chemicals. JCRB judged that HHV6 production by HUV-EC-C will not occur. Reference: Cytotechnology,70: 141-152 (2018)

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2959

Reference
Pubmed id:28755030	The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture. Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A Cytotechnology. 2018 Feb;70(1):141-152
Pubmed id:8999915	Characterization of the binding of serum amyloid P to laminin. Zahedi K J Biol Chem. 1997 Jan 24;272(4):2143-8
Pubmed id:8621443	Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-encoded domain. Soker S,Fidder H,Neufeld G,Klagsbrun M J Biol Chem. 1996 Mar 8;271(10):5761-7
Pubmed id:6333682	Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Hoshi H,McKeehan WL Proc Natl Acad Sci U S A. 1984 Oct;81(20):6413-7

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Cell No.	IFO50271	Cell Name
LOT No.	05282012	Lot Specification	distribution
Medium	Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2).	Temperature	37C
Cell Density at Seeding	3 - 6 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 39 hrs	Cell Number in Vial (cells/1ml)	7.9 x 10^5
Viability at cell freezing (%)	97.5	Antibiotics Used	free
Passage Number	P25	PDL	+13.3
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name
LOT No.	05232011	Lot Specification	distribution
Medium	Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2).	Temperature	37 C
Cell Density at Seeding	4.0 - 6.4 x 10^4 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	1.0 x 10^6
Viability at cell freezing (%)	96.2	Antibiotics Used	free
Passage Number	P25	PDL	+13
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name
LOT No.	06152010	Lot Specification	distribution
Medium	Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2).	Temperature	37 C
Cell Density at Seeding	6.6 - 8.4 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	6.3 x 10^5
Viability at cell freezing (%)	82.5	Antibiotics Used	free
Passage Number	P25	PDL	+10.6
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name
LOT No.	03092009	Lot Specification	distribution
Medium	Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2).	Temperature	37 C
Cell Density at Seeding	5 - 6 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time	46 hrs	Cell Number in Vial (cells/1ml)	6.6 x 10^5 (viable)
Viability at cell freezing (%)	88.2	Antibiotics Used	free
Passage Number	P25	PDL	+13.5
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name
LOT No.	03042008	Lot Specification	distribution
Medium	Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplment (ECGS) and 10% fetal bovine serum (GIBCO Cat#10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	approx. 7 x 10^4 cells/ml (1.4 x 10^4 cells/cm2)	Methods for Passages	0.25% trypsin and 0.02% EDTA. Split ratio 1:4.
Doubling Time	D.T. 2 - 3 days	Cell Number in Vial (cells/1ml)	6.7 x 10^5
Viability at cell freezing (%)	89	Antibiotics Used	free
Passage Number	P25	PDL	+13.5
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name
LOT No.	10312006	Lot Specification	distribution
Medium	Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (Sigma) and 10% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	5 - 10 x 10^4 cells/ml	Methods for Passages	0.05-0.25% trypsin and 0.02% EDTA
Doubling Time	1.5 - 2.0 days	Cell Number in Vial (cells/1ml)	8.9 x 10^5
Viability at cell freezing (%)	94.3	Antibiotics Used	free
Passage Number	P25	PDL	+14.1
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name
LOT No.	06092005	Lot Specification	distribution
Medium	Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement and 10% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	1 - 2 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	30 - 40 hrs	Cell Number in Vial (cells/1ml)	7.7 x 10^5
Viability at cell freezing (%)	82	Antibiotics Used	free
Passage Number	P26	PDL	13 at bank
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name
LOT No.	06252001	Lot Specification	distribution
Medium	Ham's F12K medium with 50 ug/ml ECGS (Sigma E2759), 100 ug/ml heparin and 10% FCS	Temperature	37 C
Cell Density at Seeding	2-6 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA. Growth at least additional 11 PDL was confirmed at bank.
Doubling Time		Cell Number in Vial (cells/1ml)	5.8 x 10^5
Viability at cell freezing (%)	77.1	Antibiotics Used	free
Passage Number	P25	PDL	12 at bank
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name	HUV-EC-C
LOT No.	1127	Lot Specification	distribution
Medium	Ham's F12K with 10% FBS, 100 ug/ml heparin-Ca, and 50 ug/ml ECGS.	Temperature	37 C
Cell Density at Seeding	5 x 10^4 cells/ml	Methods for Passages	trypsin-EDTA
Doubling Time	4 folds/week	Cell Number in Vial (cells/1ml)	4.6 x 10^5
Viability at cell freezing (%)	84.9	Antibiotics Used	free
Passage Number	P24	PDL	+12.4
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD (type B), MD, AST
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.)-culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name
LOT No.	10142003	Lot Specification	distribution
Medium	Ham's F12K medium with 10% fetal bovine serum, 50 ug/ml ECGS, 100 ug/ml heparin-Na salt (FBS lot; GIBCO 3762782S)	Temperature	37 C
Cell Density at Seeding	5-6 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T. = 2 days (max)	Cell Number in Vial (cells/1ml)	4.1 x 10^5
Viability at cell freezing (%)	80.3	Antibiotics Used	free
Passage Number	P25	PDL	+12.4
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture meidum	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50271	Cell Name	HUV-EC-C
LOT No.	03302015	Lot Specification	distribution
Medium	Ham's F12K medium with 10% fetal bovine serum (FBS; GIBCO Cat.# 10091), 100 ug/ml heparin and 50 ug/ml endothelial cell growth supplement	Temperature	37 C
Cell Density at Seeding	2.4 - 5.1 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 1.5 day	Cell Number in Vial (cells/1ml)	8.7 x 10^5
Viability at cell freezing (%)	86	Antibiotics Used	free
Passage Number	P25	PDL	+12.8
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11,12 D13S317:9,11 D7S820:8,12 D16S539:11,12 VWA:16 TH01:6,9.3 AM:X TPOX:8,11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - Ham's F12K	CO2 Conc.	5%
Viability immediately after thawing (%)	86	Additional information

Cell No.	IFO50271	Cell Name	HUV-EC-C
LOT No.	10192019	Lot Specification	distribution
Medium	Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2).	Temperature	37 C
Cell Density at Seeding	5.3 - 7.1 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 43 hrs	Cell Number in Vial (cells/1ml)	1.3 x 10^6
Viability at cell freezing (%)	87.9	Antibiotics Used	free
Passage Number	P24	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - Ham's F12K	CO2 Conc.	5%
Viability immediately after thawing (%)	87.9	Additional information

Cell No.	IFO50271	Cell Name	HUV-EC-C
LOT No.	08272018	Lot Specification	distribution
Medium	Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2).	Temperature	37 C
Cell Density at Seeding	3 - 7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 2.5 days	Cell Number in Vial (cells/1ml)	9.0 x 10^5
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	P24	PDL	+14.7
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - Ham's F12K	CO2 Conc.	5%
Viability immediately after thawing (%)	90	Additional information

Cell No.	IFO50271	Cell Name	HUV-EC-C
LOT No.	05232023	Lot Specification	distribution
Medium	Ham's F12K medium with 10% FBS (GIBCO Cat. # 10437, Lot 1072322), 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2).	Temperature	37 C
Cell Density at Seeding	2.4 - 9.6 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	26 - 76 hrs.	Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	96.8	Antibiotics Used	free
Passage Number	P24	PDL	+14.7
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11,12 D13S317:9,11 D7S820:8,12 D16S539:11,12 VWA:16 TH01:6,9.3 AM:X TPOX:8,11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - Ham's F12K	CO2 Conc.	5%
Viability immediately after thawing (%)	95.7	Additional information

JCRB Cell Bank

IFO50271 HUV-EC-C

Cell information

LOT Information