IFO50466 RC1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50466 | Cell Name | RC1 |
|---|---|---|---|
| Profile | This cell line produces a monoclonal antibody RC1 (IgG class). Immunochistochemically, RC1 antibody reacts with radial glia and neural progenitor cells in CNS. | Other Name | |
| Animal | mouse | Strain | |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | antibody-producing hybridoma | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | hybridoma (mouse spleen x mouse myeloma NS-1) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | round | Character | RC1 antibody, recognizing undifferentiated neural cell-specific antigen |
| Classify | hybridoma | Established by | Yamamoto, M. |
| Registered by | Yamamoto,M. | Regulation for Distribution | |
| Comment | Year | 1996 | |
| Medium | RPMI1640 medium with 15% fetal bovine serum | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | spleen x myeloma NS1 |
| Tissue Type | hybridoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
LOT Information
| Cell No. | IFO50466 | Cell Name | |
|---|---|---|---|
| LOT No. | 06282002 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10-15% fetal bovine serum (lot; BioWhittaker 0S069F) | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | D.T. = ca 24 hrs | Cell Number in Vial (cells/1ml) | 2.8 x 10^6 |
| Viability at cell freezing (%) | 90.7 | Antibiotics Used | free |
| Passage Number | Unknown (P8 at cellbank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Conirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-culutre medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50466 | Cell Name | RC1 |
|---|---|---|---|
| LOT No. | 1036 | Lot Specification | seed/distribution |
| Medium | RPMI 1640, 85% ; FBS, 15% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | dilution | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2 x 10^6 | |
| Viability at cell freezing (%) | 98 | Antibiotics Used | free |
| Passage Number | Unknown (5 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD, MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |