IFO50484 DBC1.2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50484 Cell Name   DBC1.2
Profile   This cell line is likely to be derived from olfactory dark (horizontal) basal cell. Keratin is expressed in this cell line. Other Name   
Animal   mouse Strain   C3H/He
Genus   Mus Species   musclus
Sex    Age   14.5 d fetus
Identity    Tissue for Primary Cancer   olfactory epithelium
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   dark (horizontal) basal cell-like, keratin-positive
Classify    Established by   Satoh, M. and Takeuchi, M.
Registered by   Satoh,M. Regulation for Distribution   
Comment    Year   1998
Medium   MCDB153 with 0.1mM ethanolamine, 0.1mM phosphoethanolamine,10ng/ml EGF,5ng/ml bFGF, 5ug/ml insulin,10ug/ml bovine pituitary extract and 50ug/ml transferrin. (KGM-Gold Bulletkit (Lonza 00192060) will also work.) Methods for Passages   0.1% (4,000-5,000 USP units/ml) crystalline trypsin in Ca, Mg-free PBS, and is stopped with 0.1% soybean trypsin inhibitor (Sigma type I-S).
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   nasal septum
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6534
Reference
Pubmed id:10876035Expression of neural properties in olfactory cytokeratin-positive basal cell line.
Satoh M,Yoshida T
Brain Res Dev Brain Res. 2000 Jun 30;121(2):219-22
Pubmed id:7586492Induction of NCAM expression in mouse olfactory keratin-positive basal cells in vitro.
Satoh M,Takeuchi M
Brain Res Dev Brain Res. 1995 Jul 14;87(2):111-9

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LOT Information

Cell No.   IFO50484 Cell Name   
LOT No.   07122010 Lot Specification   distribution
Medium   MCDB153 with 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine,10 ng/ml EGF, 5 ng/ml bFGF, 5 ug/ml insulin, 10 ug/ml bovine pituitary extract and 50 ug/ml transferrin Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^4 cells/mL Methods for Passages   0.1% crystalline trypsin. Trypsin treatment was terminated with 0.1% soybean trypsin inhibitor
Doubling Time   approx. 1.4 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50484 Cell Name   DBC1.2
LOT No.   1014 Lot Specification   distribution
Medium   a serum free medium based on MCDB153 (EGF, FGF, insulin, Tf, BPE, etc) Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.1% crystalline trypsin-soybean trypsin inhibitor
Doubling Time    Cell Number in Vial (cells/1ml)   8 x 10^5
Viability at cell freezing (%)   72 Antibiotics Used   free
Passage Number   P7 from cloning (>70 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50484 Cell Name   DBC1.2
LOT No.   09262023 Lot Specification   distribution
Medium   MCDB153 with 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine,10 ng/ml EGF, 5 ng/ml bFGF, 5 ug/ml insulin, 10 ug/ml bovine pituitary extract and 50 ug/ml transferrin Temperature   37 C
Cell Density at Seeding   1.6 - 4.0 x 10^4 cells/mL Methods for Passages   0.1% crystalline trypsin. Trypsin treatment was terminated with 0.1% soybean trypsin inhibitor
Doubling Time    Cell Number in Vial (cells/1ml)   9.1 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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