JCRB0024 IM-9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0024 Cell Name   IM-9
Profile   myeloma, multiple, B lymphoblastic Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   female patient with multiple myeloma. Metastasis   
Tissue Metastasized    Genetics   B lymphoblasts synthesiz. IgGk(2.4mg/10^6 cells/d).
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Immunoglobulin secreting human lymphoblast.
Classify   transformed Established by   Buell,D.N.
Registered by   Suzuki,K. Regulation for Distribution   
Comment   The cell line has receptors for growth hormone and etc. Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Subculture twice a week by dilution.
Cell Number on Passage   10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1305
Reference
Pubmed id:4359334Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture.
Gavin JR 3rd,Roth J,Neville DM Jr,de Meyts P,Buell DN
Proc Natl Acad Sci U S A. 1974 Jan;71(1):84-8

Images



LOT Information

Cell No.   JCRB0024 Cell Name   
LOT No.   03302009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Simple dilution, passage twice a week.
Doubling Time   approx. 23 hrs Cell Number in Vial (cells/1ml)   5.8 x 106
Viability at cell freezing (%)   88.3 Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0024 Cell Name   IM-9
LOT No.   02262009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   0.18-1.2x10^5 cells/sq.cm Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.94x10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9,11
D7S820:11,12
D16S539:9,13
VWA:14,17
TH01:6,9.3
AM:X
TPOX:11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0024 Cell Name   
LOT No.   09172008 Lot Specification   distribution
Medium   RPM 1640 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   3 - 6 x 10^5 cells/ml Methods for Passages   Simple dilution
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   6.3 x 10^6
Viability at cell freezing (%)   82.4 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0024 Cell Name   IM-9
LOT No.   101293 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (JRS, DO10400) Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   Simply dilute to fresh medium at the concentration above.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.74x10^6
Viability at cell freezing (%)   71.5 Antibiotics Used   free
Passage Number   P6* (P42 on ampule) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   from lot.052785 by Ohnishi,K.
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0024 Cell Name   IM-9
LOT No.   100690 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Subculture twice a week by dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:9,11
D7S820:11,12
D16S539:9,13
VWA:14,17
TH01:6,10
AM:X
TPOX:11
CSF1PO:10,11
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0024 Cell Name   IM-9
LOT No.   030689 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Subculture twice a week by dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0024 Cell Name   IM-9
LOT No.   041186 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Subculture twice a week by dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0024 Cell Name   
LOT No.   091098 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 Methods for Passages   Dilution
Doubling Time    Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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