JCRB0066 Mewo

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0066 Cell Name   Mewo
Profile   malignant melanoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   78
Identity   available Tissue for Primary Cancer   melanoma, skin
Case history   malignant melanoma Metastasis   Y
Tissue Metastasized   lymph node Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Malignant melanoma (skin). Established from a melanotic lymph node metastasis. Slightly produces melanin.
Classify   tumor Established by   Bean,M.A.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/8 Race   
CO2 Conc.   5 % Tissue Sampling   lymph node
Tissue Type   malignant melanoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0445
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 908128
Reference
Pubmed id:2121721Monoclonal antibodies to disialogangliosides: characterization of antibody-mediated cytotoxicity against human melanoma and neuroblastoma cells in vitro.
Kawashima I,Tada N,Fujimori T,Tai T
J Biochem. 1990 Jul;108(1):109-15
Pubmed id:2736210Production of transforming growth factor-alpha in human tumour cell lines.
Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K
Br J Cancer. 1989 May;59(5):761-5
Pubmed id:978138Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens.
Shiku H,Takahashi T,Oettgen HF
J Exp Med. 1976 Oct 1;144(4):873-81


Research results by users. Click！
Pubmed id:27595314 Stability and Bioavailability of Lentztrehaloses A, B, and C as Replacements for Trehalose.
Wada S,Sawa R,Ohba S,Hayashi C,Umekita M,Shibuya Y,Iijima K,Iwanami F,Igarashi M
J Agric Food Chem. 2016 Sep 28;64(38):7121-6
Pubmed id:2733038 Enhanced tumorigenicity, melanogenesis, and metastases of a human malignant melanoma after subdermal implantation in nude mice.
Cornil I,Man S,Fernandez B,Kerbel RS
J Natl Cancer Inst. 1989 Jun 21;81(12):938-44

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LOT Information

Cell No.   JCRB0066 Cell Name   
LOT No.   04162002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   6-9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   D.T. = ca 44 hrs Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0066 Cell Name   Mewo
LOT No.   052493 Lot Specification   distribution
Medium   Eagle's minimal essential medium with Non-essential amino acids(GIBCO) and 10% FBS heat inactivated. Temperature   37 C
Cell Density at Seeding   1:8 dilution Methods for Passages   Harvest cells by 0.02% EDTA and 0.2% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.2x10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   P24 PDL   *
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0066 Cell Name   Mewo
LOT No.   081391 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   95% Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0066 Cell Name   Mewo
LOT No.   112789 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0066 Cell Name   Mewo
LOT No.   061587 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8x10^5
Viability at cell freezing (%)   92% Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0066 Cell Name   Mewo
LOT No.   01122015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
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