JCRB0070 MIA PaCa-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0070 Cell Name   MIA PaCa-2
Profile   pancreatic cancer Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   65
Identity   available Tissue for Primary Cancer   pancreas
Case history   pancreatic cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   sensitive to asparginase. Dt=40hr, colony-formaing efficiency in soft agar = 19%
Classify   tumor Established by   Yunis,A. et al.
Registered by   Akiyama,M. Regulation for Distribution   free
Comment    Year   1985
Medium   Eagle's minimal essential medium with non-essential a.a. and 10% fetal calf serum(heat inactivated) Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/3 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   pancreas
Tissue Type   pancreatic cancer

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0428
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 724870
Reference
Pubmed id:15590Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells.
Wu M,Arimura GK,Yunis AA
Biochemistry. 1977 May 3;16(9):1908-13
Pubmed id:832918Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.
Yunis AA,Arimura GK,Russin DJ
Int J Cancer. 1977 Jan;19(1):128-35

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LOT Information

Cell No.   JCRB0070 Cell Name   
LOT No.   11162009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids (0.1 mM) and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   20-23 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   P154 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B). MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0070 Cell Name   
LOT No.   04232001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   2-5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   77 Antibiotics Used   free
Passage Number   P155 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0070 Cell Name   MIA PaCa-2
LOT No.   092493 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1x10^6 cells/10 cm plate Methods for Passages   Cells are treated with 0.05% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   2.14x10^6
Viability at cell freezing (%)   79.0 Antibiotics Used   free
Passage Number   P151 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0070 Cell Name   MIA PaCa-2
LOT No.   050891 Lot Specification   distribution
Medium   MEM with NEAA + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 1/3 - 1/4 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P153 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0070 Cell Name   MIA PaCa-2
LOT No.   082889 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 - 1/4 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P152 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0070 Cell Name   MIA PaCa-2
LOT No.   033187 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 - 1/4 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P151 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0070 Cell Name   MIA PaCa-2
LOT No.   01272017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.4 - 2.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 22 hrs Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   95.8 Antibiotics Used   free
Passage Number   P154 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Cell No.   JCRB0070 Cell Name   MIA PaCa-2
LOT No.   11132020 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-080) with non essential amino acid (GIBCO cat.No.11140-050) and 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   1.91-4.32x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.42x10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   p154 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:12,13
D7S820:12,13
D16S539:10,13
VWA:15
TH01:9,10
AM:X
TPOX:9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   85.9 Additional information   
Images
Cell No.   JCRB0070 Cell Name   MIA PaCa-2
LOT No.   07072022 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   P154 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:12,13
D7S820:12,13
D16S539:10,13
VWA:15
TH01:9,10
AM:X
TPOX:9
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   96.9 Additional information   
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