JCRB0072 MiTen
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0072 | Cell Name | MiTen |
|---|---|---|---|
| Profile | Human cell line from Lesch-Nyhan syndrome. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Lesh-Nyhan syndrome, genetic disease | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | normal | Established by | Lesch,M. & Nyhan,W. |
| Registered by | Akiyama,M. | Regulation for Distribution | |
| Comment | Year | ||
| Medium | Eagle's minimal essentila medium with 10% fetal calf serum | Methods for Passages | Treat cells with 0.25 % trypsin twice a week |
| Cell Number on Passage | split ratio=1/2-1/3 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type | normal skin fibroblast |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | NT | ||||||
| Notes | |||||||||
| Images |
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LOT Information
| Cell No. | JCRB0072 | Cell Name | MiTen |
|---|---|---|---|
| LOT No. | 082391 | Lot Specification | distribution |
| Medium | MEM + 10 % FCS | Temperature | 37 C |
| Cell Density at Seeding | dilution 1/2-1/3 | Methods for Passages | 0.25 % trypsin, twice a week |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 8.2x10^5 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P9 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |