JCRB0079 LU65

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0079 Cell Name   LU65
Profile   Human cell line with giant cell carcinoma of lung. Other Name   LU65
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung giant cell carcinoma Metastasis   
Tissue Metastasized    Genetics   c-Ki-ras2 mut. codon 12. ({3601,3602})
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   many floating cells are observed
Classify   tumor Established by   Hirohashi,S. & Simosato,Y.
Registered by   Suzuki,K. Regulation for Distribution   
Comment    Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1392
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 724863
Reference
Pubmed id:2197591Detection of ras gene mutations in human lung cancers by single-strand conformation polymorphism analysis of polymerase chain reaction products.
Suzuki Y,Orita M,Shiraishi M,Hayashi K,Sekiya T
Oncogene. 1990 Jul;5(7):1037-43
Pubmed id:2659722Purification and partial primary sequence of a chemotactic protein for polymorphonuclear leukocytes derived from human lung giant cell carcinoma LU65C cells.
Suzuki K,Miyasaka H,Ota H,Yamakawa Y,Tagawa M,Kuramoto A,Mizuno S
J Exp Med. 1989 Jun 1;169(6):1895-901
Pubmed id:3778442Establishment of a human pancreatic adenocarcinoma cell line (PSN-1) with amplifications of both c-myc and activated c-Ki-ras by a point mutation.
Yamada H,Yoshida T,Sakamoto H,Terada M,Sugimura T
Biochem Biophys Res Commun. 1986 Oct 15;140(1):167-73
Pubmed id:3935627Giant cell carcinomas of the lung producing colony-stimulating factor in vitro and in vivo.
Yamada T,Hirohashi S,Shimosato Y,Kodama T,Hayashi S,Ogura T,Gamou S,Shimizu N
Jpn J Cancer Res. 1985 Oct;76(10):967-76
Pubmed id:6098458A novel combination of K-ras and myc amplification accompanied by point mutational activation of K-ras in a human lung cancer.
Taya Y,Hosogai K,Hirohashi S,Shimosato Y,Tsuchiya R,Tsuchida N,Fushimi M,Sekiya T,Nishimura S
EMBO J. 1984 Dec 1;3(12):2943-6

Images



LOT Information

Cell No.   JCRB0079 Cell Name   
LOT No.   12162008 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   1.3 - 4.7 x 10^5 cells/ml Methods for Passages   Most cells are in suspension. Passage floating cells as well as attached cells by pipetting and dilution.
Doubling Time   35 - 40 hrs Cell Number in Vial (cells/1ml)   93.5 x 10^6
Viability at cell freezing (%)   91.4 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0079 Cell Name   LU65
LOT No.   101093 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat inactivated fetal bovine serum (JRS.DO10400) Temperature   37 C
Cell Density at Seeding   1.5x10^5 cells/ml Methods for Passages   Cells are simply diluted to the fresh medium.
Doubling Time   21.7 hrs Cell Number in Vial (cells/1ml)   1.88x10^6
Viability at cell freezing (%)   86.2 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0079 Cell Name   LU65
LOT No.   052791 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA and 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0079 Cell Name   LU65
LOT No.   070489 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA and 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.9x10^6
Viability at cell freezing (%)   97% Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0079 Cell Name   LU65
LOT No.   041486 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA and 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   0.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0079 Cell Name   LU65
LOT No.   01192016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   2.1 - 3.0 x 10^5 cells/mL Methods for Passages   Most of cells was round morphology and could be detached by pipetting, but some cells firmly attached to dishes and were treated with 0.1% trypsin.
Doubling Time   approx. 46 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   
Cell No.   JCRB0079 Cell Name   LU65
LOT No.   01192022 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/mL Methods for Passages   Most of cells was round morphology and could be detached by pipetting, but some cells firmly attached to dishes and were treated with 0.05% trypsin.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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