JCRB0081 RERF-LC-MS

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0081 Cell Name   RERF-LC-MS
Profile   adenocarcinoma Other Name   old name erased
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history    Metastasis   
Tissue Metastasized    Genetics   adenocarcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   lung adenocarcinoma
Classify   tumor Established by   Akiyama,M
Registered by   Akiyama,M Regulation for Distribution   free
Comment    Year   1985
Medium   Eagle's minimal essential medium with non-essential amino acids(GIBCO 100x) & 10% fetal bovine serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1655
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910931
Reference
Pubmed id:4005855Monoclonal antibodies to human squamous cell carcinoma of the lung and their application to tumor diagnosis.
Kyoizumi S,Akiyama M,Kouno N,Kobuke K,Hakoda M,Jones SL,Yamakido M
Cancer Res. 1985 Jul;45(7):3274-81

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LOT Information

Cell No.   JCRB0081 Cell Name   
LOT No.   08102009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 0.1 mM non-essential amino acids and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   4 - 6 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   30 - 38 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   97.3 Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0081 Cell Name   RERF-LC-MS
LOT No.   053097 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (JRH Bio Temperature   37 C
Cell Density at Seeding   1.8x10^4 cells/sq.cm. Methods for Passages   Cells were washed with PBS(-) once and treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   P9 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:10
D7S820:11,12
D16S539:12
VWA:16
TH01:7
AM:XY
TPOX:8,11
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0081 Cell Name   RERF-LC-MS
LOT No.   012194 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids(GIBCO100x) & 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   Floating cells were harvested & then plates were treated with 0.25% trypsin & 0.02% EDTA for 5-10min
Doubling Time   dt=42.7 hrs Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   93.4 Antibiotics Used   free
Passage Number   P9 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD, PepB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0081 Cell Name   RERF-LC-MS
LOT No.   030291 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA + 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.5x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0081 Cell Name   RERF-LC-MS
LOT No.   072187 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA + 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0081 Cell Name   RERF-LC-MS
LOT No.   10282020 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-080) with non essential amino acid (GIBCO cat.No.11140-050) and 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   5.16-6.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.91x10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   p10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:10
D7S820:11,12
D16S539:12
VWA:16
TH01:7
AM:X,Y
TPOX:8,11
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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