JCRB0095 P32/ISH

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0095 Cell Name   P32/ISH
Profile   lymphoma, Burkitt's Other Name   Ishida
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   8
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   Burkitt lymphoma Metastasis   
Tissue Metastasized    Genetics   B cell characteristics,EBNA-, Dt=40hr
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   translocation between chr.2 and chr.14, Ig gene
Classify   tumor Established by   Hirose,M.
Registered by   Minato,K. Regulation for Distribution   No commercial purpose. Permission required.
Comment   Good for the research of Ig gene. because of the chromosome translocation (2,14). Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Simple dilution.
Cell Number on Passage   split ratio=1/5 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes EBV(low copy)

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3119
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1330987
Reference
Pubmed id:6667834A novel Japanese Burkitt's lymphoma cell line, P32/Ishida, with a new variant chromosomal translocation (2;14).
Hirose M,Minato K,Tobinai K,Ise T,Watanabe S,Shimoyama M,Abe T
Gan. 1983 Dec;74(6):878-86

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LOT Information

Cell No.   JCRB0095 Cell Name   
LOT No.   02122004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS Lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1.5 - 2.0 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   ca. 1.3 days Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   89.2 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0095 Cell Name   P32/ISH
LOT No.   110691 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   5.75x10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.75x10^6
Viability at cell freezing (%)   79.% Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0095 Cell Name   P32/ISH
LOT No.   100386 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/5 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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