JCRB0097 A4/Fuk

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0097 Cell Name   A4/Fuk
Profile   lymphoma, malignant, B-cell, non-Hodgkin's, IgM kappa-producing Other Name   A4/Fuk
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   52
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   malignant lymphoma at ileocecal Metastasis   
Tissue Metastasized    Genetics   ER-,PanT-,CD4+,PanB-,J5-,CD11-,CIg+,SIg+,TdT-,Ia+
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   lymphoma cell line producing IgM,k, Dt=30hr
Classify   tumor Established by   Kaneda
Registered by   Minato,K. Regulation for Distribution   No commercial purpose. Permission required.
Comment   Mycoplasma contamination was detected when submitted to NIHS and eliminated in 1986. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Subculture by mechanical pipetting.
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1064
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910934
Reference
Pubmed id:6601597Two novel cultured cell lines, A3/Kawakami and A4/Fukuda, derived from malignant lymphoma of B(non-T)-cell nature of the gastrointestinal tract.
Hirose M,Minato K,Tobinai K,Shimoyama M,Watanabe S,Abe T,Deura K
Gan. 1983 Feb;74(1):106-15

Images



LOT Information

Cell No.   JCRB0097 Cell Name   
LOT No.   02162007 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   1.5-2.2 x 10^5 cells/ml Methods for Passages   dilution, subculture twice a week.
Doubling Time   38-43 hrs Cell Number in Vial (cells/1ml)   4.8 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP and G6PD, LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0097 Cell Name   A4/Fuk
LOT No.   110591 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% DO101591 Temperature   37
Cell Density at Seeding   1.25x10^6 cells/ml Methods for Passages   Dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1x10^6/ml
Viability at cell freezing (%)   89.5 Antibiotics Used   free
Passage Number   P15* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0097 Cell Name   A4/Fuk
LOT No.   05202014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time    Cell Number in Vial (cells/1ml)   8.9 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:9,12
D7S820:8,11
D16S539:9,12
VWA:18,19,20
TH01:7,9
AM:X
TPOX:11
CSF1PO:11,13
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   
Cell No.   JCRB0097 Cell Name   A4/Fuk
LOT No.   08052021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 37 hrs. Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   84.7 Antibiotics Used   fre
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   82.8 Additional information   
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