JCRB0098 KURAMOCHI

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0098 Cell Name   KURAMOCHI
Profile   undifferentiated carcinoma, ascites Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   unk
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian cancer, undifferentiated carcinoma Metastasis   
Tissue Metastasized    Genetics   hyperdiploid
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Dt=24-27hr
Classify   tumor Established by   Kaneda
Registered by   Minato,K. Regulation for Distribution   
Comment   Mycoplasma contamination was detected when submitted to NIHS and eliminated at 1986. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1345
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909975
Reference
Pubmed id:7069245[Quantitative analysis on in vitro drug sensitivity of cultured human ovarian cancer cell lines (author's transl)].
Motoyama T
Nihon Sanka Fujinka Gakkai Zasshi. 1982 Mar;34(3):308-14

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LOT Information

Cell No.   JCRB0098 Cell Name   
LOT No.   10242008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   60 hrs Cell Number in Vial (cells/1ml)   6.2 x 10^6
Viability at cell freezing (%)   95.8 Antibiotics Used   free
Passage Number   Unknown (17 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   090298 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Mitsubishi PVF01). Temperature   37 C
Cell Density at Seeding   1.8 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA for 5 minutes at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P16* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP and LDH examined. human, non-HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:9,12
D7S820:10,11
D16S539:10
VWA:16,19
TH01:9
AM:X
TPOX:8,12
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   082289 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02 % EDTA and 0.25 % tripsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P16* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   12132013 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37C
Cell Density at Seeding   0.6 - 1.2 x 10^5 cells/mL Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   2.1 x 10^6 cells/1mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS- RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   94.5 Additional information   
Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   06302015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.3 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 43 hrs. Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   93.4 Antibiotics Used   free
Passage Number   P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:9,12
D7S820:10,11
D16S539:10
VWA:16,19
TH01:9
AM:X
TPOX:8,12
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   93.4 Additional information   
Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   06152016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.5 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 40 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   81.9 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI-1640 CO2 Conc.   5%
Viability immediately after thawing (%)   81.9 Additional information   
Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   09042020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.6 - 2.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknlown (17 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   10262022 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   6.2 - 9.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:9,12
D7S820:10,11
D16S539:10
VWA:16,19
TH01:9
AM:X
TPOX:8,12
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   88 Additional information   
Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   12052023 Lot Specification   distribution
Medium   MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids (Nacalai Tesque #21443-15) with 10% heat-inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.5-4.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   p17* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:9,12
D7S820:10,11
D16S539:10
VWA:16,19
TH01:9
AM:X
TPOX:8,12
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   92.0 Additional information   
Cell No.   JCRB0098 Cell Name   KURAMOCHI
LOT No.   12062023 Lot Specification   distribution
Medium   MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids (Nacalai Tesque #21443-15) with 10% heat-inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.5-4.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^5
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   p17* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:9,12
D7S820:10,11
D16S539:10
VWA:16,19
TH01:9
AM:X
TPOX:8,12
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94.2 Additional information   
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