JCRB0153.1 tsJT663
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0153.1 | Cell Name | tsJT663 |
|---|---|---|---|
| Profile | Rat embryo cell line with ts mutation for cell cycle progression from G1 to S phase. | Other Name | tsJT663(3Y1) |
| Animal | rat | Strain | Fischer 344 |
| Genus | Rattus | Species | norvegicus |
| Sex | F | Age | fetus |
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | A ts mutant for cell cycle progression from G1 to S phase. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | MNNG-mutagenized 3Y1 cell derivatives with ts mutation grows at 34 C but the growth is arrested at 40 C. |
| Classify | mutant | Established by | Nihomiya-Tsuji,J. and Ide.T. |
| Registered by | Kyoizumi,S. | Regulation for Distribution | free |
| Comment | Year | 2000 | |
| Medium | Dulbecco's modified Eagle's medium with 0.1 % glucose and 10% fetal calf serum. | Methods for Passages | Cells are harvested after treatment with 0.125% trypsin and 0.01% EDTA. Gentle piptting may be reauired to detach cells from dishes. Subculture once a week at split ratio=1/8 at 34 C. |
| Cell Number on Passage | 5-8 x 10^5 cells/60mm dish | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | embryo |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:8082723 | Isolation of a mammalian cell cycle ts mutant, tsJT663, defective in preparation for passing restriction point. Sasaki T,Kameoka Y,Kamase S,Ide T Exp Cell Res. 1994 Sep;214(1):209-14 |
| Images |
|---|
             |
LOT Information
| Cell No. | JCRB0153.1 | Cell Name | tsJT663 |
|---|---|---|---|
| LOT No. | 05022001 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB52405). | Temperature | 34 C (ts) |
| Cell Density at Seeding | 7.0 x 10^3 cells/sq.cm. | Methods for Passages | Cells harvesed after treatment with 0.125% trypsin and 0.01% EDTA. Subculture once a week and medium change twice a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.2 x 10^6 |
| Viability at cell freezing (%) | 97.5 | Antibiotics Used | free |
| Passage Number | P8* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Isozyme LDH, NP, G6PD. Rat. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
|---|
|
|