JCRB0169 Lu-134-B
Cell information
Important Notice(s)Culture medium for JCRB0169 Lu-134-B
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0169 | Cell Name | Lu-134-B |
|---|---|---|---|
| Profile | Small cell lung carcinoma cell line established through heterotransplanted tumor. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 80 |
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | small cell carcinoma arising in the periphery of right lower lobe of lung. | Metastasis | |
| Tissue Metastasized | Genetics | Activities of gamma-enolase and aromatic L-amino acid decarboxylase are high significantly. Hypertetraploid chromosome pattern. M=85, Td=85 hrs | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Cluster formation. | Character | Derived from small cell lung carcinoma, classic type. Cells are prepared from heterotransplantation of the tumor into athymic BALB/c mice. Dense cored granule (neurosecretory granule) observed unde EM. |
| Classify | tumor | Established by | Terasaki,T. and Shimosato,Y. |
| Registered by | Terasaki,T. | Regulation for Distribution | |
| Comment | Year | 2001 | |
| Medium | Eagle's minimal essential medium supplemented with glutamine and 10% heat-inactivated fetal calf serum. | Methods for Passages | Same volume of a fresh medium as the cell culture was added to the cell suspension and pipetting twice or three times. Take a portion of the diluted cell suspension is applied to further cell culture. |
| Cell Number on Passage | split ratio = 1/2. | Race | Japanse |
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2168867 | Selection of radioresistant cells by vitamin A deficiency in a small cell lung cancer cell line. Terasaki T,Shimosato Y,Wada M,Yokota J,Terada M Jpn J Cancer Res. 1990 Aug;81(8):780-5 |
| Pubmed id:2438038 | Reversible squamous cell characteristics induced by vitamin A deficiency in a small cell lung cancer cell line. Terasaki T,Shimosato Y,Nakajima T,Tsumuraya M,Ichinose H,Nagatsu T,Kato K Cancer Res. 1987 Jul 1;47(13):3533-7 |
| Pubmed id:6098504 | Interconversion of biological characteristics of small cell lung cancer depending on culture conditions. Terasaki T,Kameya T,Nakajima T,Tsumuraya M,Shimosato Y,Kato K,Ichinose H,Nagatsu T,Hasegawa T Gan. 1984 Dec;75(12):1089-99 |
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LOT Information
| Cell No. | JCRB0169 | Cell Name | |
|---|---|---|---|
| LOT No. | 06022003 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) | Temperature | 37 C |
| Cell Density at Seeding | uncountable due to aggregates. Split ratio 1:6-1:3 | Methods for Passages | Dilution with pipetting large cell aggregates to small fragments. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 5 - 10 x 10^6 | |
| Viability at cell freezing (%) | ca. 70 | Antibiotics Used | free |
| Passage Number | > P31 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD and LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |