JCRB0178.1 KP-3L
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0178.1 | Cell Name | KP-3L |
|---|---|---|---|
| Profile | Human tumor cell line obtained from metastasis of adenosquamous carcinoma of the pancreatic duct. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 75 |
| Identity | available | Tissue for Primary Cancer | pancreas |
| Case history | pancreatic tumor | Metastasis | Yes |
| Tissue Metastasized | liver | Genetics | Tissue was classified as a metastasis of adenosquamous carcinoma of the pancreatic duct. |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Cells were established from the resulting tumor nodules in the liver of mouse, into the spleen where KP-3 cells had been injected. |
| Classify | tumor | Established by | Kono,A. et.al. |
| Registered by | Iguchi,H. | Regulation for Distribution | |
| Comment | Mycoplasmas were decontaminated by the MC210 treatment in the JCRB cell bank. | Year | 2001 |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | Cells are harvested after treatment with 0.02% EDTA and 0.1% trypsin. Subculture cells when they reach to subconfluent. |
| Cell Number on Passage | 1-2 x 10^5 cells/T25 frask | Race | Japanese |
| CO2 Conc. | Tissue Sampling | pancreas | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:9735394 | Alteration of the CDKN2A gene in pancreatic cancers: Is it a late event in the progression of pancreatic cancer? Sugimoto Y,Morita R,Hikiji K,Imura G,Ogata Y,Yasuda D,Kono A,Iguchi H Int J Oncol. 1998 Oct;13(4):669-76 |
| Pubmed id:21607521 | Alterations of the p53 tumor-suppressor gene and ki-ras oncogene in human pancreatic cancer-derived cell-lines with different metastatic potential. Iguchi H,Morita R,Yasuda D,Takayanagi R,Ikeda Y,Takada Y,Shimazoe T,Nawata H,Kono A Oncol Rep. 1994 Nov;1(6):1223-7 |
| Pubmed id:2172194 | Establishment and characterization of human pancreatic cancer cell lines in tissue culture and in nude mice. Ikeda Y,Ezaki M,Hayashi I,Yasuda D,Nakayama K,Kono A Jpn J Cancer Res. 1990 Oct;81(10):987-93 |
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LOT Information
| Cell No. | JCRB0178.1 | Cell Name | |
|---|---|---|---|
| LOT No. | 06292006 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) | Temperature | 37 C |
| Cell Density at Seeding | 1.0-1.4 x 10^5 cells/ml | Methods for Passages | 0.1% trypsin and 0.02% EDTA |
| Doubling Time | approx. 2 days | Cell Number in Vial (cells/1ml) | 4.4 x 10^6 |
| Viability at cell freezing (%) | 96.1 | Antibiotics Used | free |
| Passage Number | Unknown (12 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0178.1 | Cell Name | KP-3L |
|---|---|---|---|
| LOT No. | 11132001 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat-inactivated fetal calf serum (Intergen RB52405). | Temperature | 37 C |
| Cell Density at Seeding | 2.3 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested after treatment with 0.1% trypsin and 0.02% EDTA-PBS. Medium change 3 times a week. Subculture once two weeks. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 96.3 | Antibiotics Used | free |
| Passage Number | P9* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), NP, LDH examined. Human not HeLa. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:13 D13S317:9,11 D7S820:10,12 D16S539:9,13 VWA:17,18 TH01:6,10 AM:X TPOX:9,11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0178.1 | Cell Name | KP-3L |
|---|---|---|---|
| LOT No. | 03222016 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). | Temperature | 37 C |
| Cell Density at Seeding | 3.4 - 5.8 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 34 hrs | Cell Number in Vial (cells/1ml) | 3.6 x 10^6 |
| Viability at cell freezing (%) | 80.6 | Antibiotics Used | free |
| Passage Number | Unknown (12 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 80.6 | Additional information |