JCRB0227 C32TG

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0227 Cell Name   C32TG
Profile   Human 6TG resistant amelanotic melanoma cell line from skin. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   melanoma
Case history    Metastasis   
Tissue Metastasized    Genetics   amelanotic melanoma, 6TG-r
Life Span   infinite Crisis PDL   
Morphology    Character   amelanotic melanoma, 6TG-r
Classify    Established by   Chen,T.R.
Registered by   Egashira, Y. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2324
Reference
Pubmed id:277716Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line.
Chen TR
J Natl Cancer Inst. 1978 Aug;61(2):277-84
Pubmed id:4725368Stable chromosome changes in human malignant melanoma.
Chen TR,Shaw MW
Cancer Res. 1973 Sep;33(9):2042-7

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LOT Information

Cell No.   JCRB0227 Cell Name   C32TG
LOT No.   09262006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   3 x 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   37 hrs Cell Number in Vial (cells/1ml)   8.6 x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:12
D7S820:9,11
D16S539:11,12
VWA:14,19
TH01:9.3
AM:X,Y
TPOX:9
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS- EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0227 Cell Name   
LOT No.   122282 Lot Specification   distribution
Medium   MEM + FCS 10 % Temperature   37
Cell Density at Seeding    Methods for Passages   0.25 % trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0227 Cell Name   C32TG
LOT No.   10302018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1 - 4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 43 hrs. Cell Number in Vial (cells/1ml)   9.6 x 10^5
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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