JCRB0234.0 TYK-nu Cell information
Cell type:general cells
(View Pricing Information )
JCRB No.
JCRB0234.0
Cell Name
TYK-nu
Profile
undifferentiated carcinoma
Other Name
Animal
human
Strain
Genus
Homo
Species
sapiens
Sex
Age
Identity
available
Tissue for Primary Cancer
ovary
Case history
Metastasis
Tissue Metastasized
Genetics
ovary, undifferentiated carcinoma
Life Span
Crisis PDL
Morphology
Character
ovary, undifferentiated carcinoma
Classify
Established by
Yoshiya,N.
Registered by
Egashira, Y.
Regulation for Distribution
Make notice to the originator on distribution.
Comment
Year
1987
Medium
Eagle's minimal essential medium with 10% fetal calf serum
Methods for Passages
Cells are treated with 0.25 % trypsin, 0.1-0.01 % EDTA (4/1)
Cell Number on Passage
2x10^4 cells/ml
Race
CO2 Conc.
5 %
Tissue Sampling
ovary
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
tested
Detected RNA Virus
tested
CMV
-
parvoB19
-
HCV
-
HTLV-1
-
EBV
-
HBV
-
HIV-1
-
HTLV-2
-
HHV6
-
HTLV-1
-
HIV-2
-
HAV
-
HHV7
-
HTLV-2
-
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV
-
HIV-1
-
JCV
-
HIV-2
-
ADV
-
HPV18
-
Notes
Reference
Pubmed id:3782955 [Establishment of a cell line from human ovarian cancer (undifferentiated carcinoma of FIGO classification) and analysis of its cell-biological characteristics and sensitivity to anticancer drugs]. Yoshiya N Nihon Sanka Fujinka Gakkai Zasshi. 1986 Oct;38(10):1747-53
LOT Information
Cell No.
JCRB0234.0
Cell Name
LOT No.
012099
Lot Specification
distribution
Medium
Eagle's minimal essential medium + 10% fetal calf serum (Biowhittaker 6M0121)
Temperature
37 C
Cell Density at Seeding
5 x 10^4 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA
Doubling Time
ca 50hrs
Cell Number in Vial (cells/1ml)
1.8 X10^6
Viability at cell freezing (%)
86%
Antibiotics Used
free
Passage Number
P9
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0234.0
Cell Name
LOT No.
100794
Lot Specification
distribution
Medium
MEM + 10 % FCS
Temperature
37
Cell Density at Seeding
1/5 - 1/20
Methods for Passages
0.25% trypsin, 0.02% EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
1.1 x 10^6
Viability at cell freezing (%)
96.0
Antibiotics Used
free
Passage Number
P106
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
AST, G6PD, LD, MD, MPI, NP, PEPB
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0234.0
Cell Name
TYK-nu
LOT No.
07182013
Lot Specification
distribution
Medium
Eagle's minimal essential medium with 10% fetal calf serum (FBS; GIBCO Cat. # 10099)
Temperature
37C
Cell Density at Seeding
6.4 x 10^4 cells/mL
Methods for Passages
Cells are treated with 0.25 % trypsin, 0.02% EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
2.2 x 10^6 (cells/1
Viability at cell freezing (%)
Antibiotics Used
free
Passage Number
P12
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
5%
Viability immediately after thawing (%)
92.4
Additional information
Cell No.
JCRB0234.0
Cell Name
TYK-nu
LOT No.
08192016
Lot Specification
distribution
Medium
Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)
Temperature
37 C
Cell Density at Seeding
1.0 - 1.5 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 44 hrs.
Cell Number in Vial (cells/1ml)
1.7 x 10^6
Viability at cell freezing (%)
83
Antibiotics Used
free
Passage Number
P12
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - EMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
83
Additional information
Cell No.
JCRB0234.0
Cell Name
TYK-nu
LOT No.
02252019
Lot Specification
distribution
Medium
Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)
Temperature
37 C
Cell Density at Seeding
0.5 - 1.5 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 26 hrs.
Cell Number in Vial (cells/1ml)
3.4 x 10^6
Viability at cell freezing (%)
95
Antibiotics Used
free
Passage Number
P12
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - EMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
90
Additional information
Cell No.
JCRB0234.0
Cell Name
TYK-nu
LOT No.
02132023
Lot Specification
distribution
Medium
Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437)
Temperature
37 C
Cell Density at Seeding
0.4-1.2 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
28 - 34 hrs.
Cell Number in Vial (cells/1ml)
2.3 x 10^6
Viability at cell freezing (%)
98.1
Antibiotics Used
free
Passage Number
P12
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
5% DMSO, 20% FBS - EMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
95.1
Additional information