JCRB0236 KG-1-C
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0236 | Cell Name | KG-1-C |
|---|---|---|---|
| Profile | mixed glioma, GFAP-negative, S-100-positive (growth is very slow). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 13 |
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | mixed glioma | |
| Life Span | Crisis PDL | ||
| Morphology | Character | mixed glioma | |
| Classify | Established by | Miyake,E. | |
| Registered by | Egashira, Y. | Regulation for Distribution | |
| Comment | Year | 1987 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum | Methods for Passages | 200 units trypsin, 0.05 % EDTA |
| Cell Number on Passage | 5x10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | brain |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:4093122 | [Establishment of a human single cell clonal line synthesizing nervous tissue specific protein (S-100 protein)]. Miyake E Fukuoka Igaku Zasshi. 1985 Oct;76(10):500-7 |
| Pubmed id:222111 | Establishment of a human oligodendroglial cell line. Miyake E Acta Neuropathol. 1979 Apr 12;46(1-2):51-5 |
| Images |
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LOT Information
| Cell No. | JCRB0236 | Cell Name | |
|---|---|---|---|
| LOT No. | 040292 | Lot Specification | distribution |
| Medium | DMEM + 20 % FCS | Temperature | 37 |
| Cell Density at Seeding | 5x10^4/cm2 | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3.0 x 10^5 | |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | *P9 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | AST, G6PD, LD, MD, MPI, NP, PEPB |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0236 | Cell Name | KG-1-C |
|---|---|---|---|
| LOT No. | 041698 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 4-6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | 5-6 days | Cell Number in Vial (cells/1ml) | 4.7 x 10^5 |
| Viability at cell freezing (%) | 85.0 | Antibiotics Used | free |
| Passage Number | Unknown (13 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0236 | Cell Name | KG-1-C |
|---|---|---|---|
| LOT No. | 03012016 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 1.0 - 6.8 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | Very slow (D.T.=3.5 - 5 days) | Cell Number in Vial (cells/1ml) | 9.0 x 10^5 |
| Viability at cell freezing (%) | 94.8 | Antibiotics Used | free |
| Passage Number | Unknown (12 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS -DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94.8 | Additional information |