JCRB0251 SCH

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0251 Cell Name   SCH
Profile   choriocarcinoma arising at stomach from male patient Other Name   SCH
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   stomach
Case history    Metastasis   
Tissue Metastasized    Genetics   stomach, choriocarcinoma, 46 year-old male
Life Span    Crisis PDL   
Morphology    Character   stomach, choriocarcinoma, 46 year-old male
Classify    Established by   Ohboshi,S.
Registered by    Regulation for Distribution   
Comment   Mycoplasmas eliminated by MC-210 in FDSC. Year   1989
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25 % trypsin and 0.02 % EDTA.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1687
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909711
Reference
Pubmed id:3095279Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel.
Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M
Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:1170940A human gastric choriocarcinoma cell line with human chorionic gonadotropin and placental alkaline phosphatase production.
Kameya T,Kuramoto H,Suzuki K,Kenjo T,Oshikiri T,Hayashi H,Itakura M
Cancer Res. 1975 Aug;35(8):2025-32
Pubmed id:5058640Use of 1-nitroso-2-naphthol in quantitative determination of medicinal phenolic compounds.
Bhansali KG
J Pharm Sci. 1972 Jan;61(1):146-7

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LOT Information

Cell No.   JCRB0251 Cell Name   
LOT No.   07022010 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.3 - 1.7 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 1.5 days. Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0251 Cell Name   
LOT No.   102594 Lot Specification   distribution
Medium   RPMI1640 + FCS 10 % Temperature   37
Cell Density at Seeding    Methods for Passages   0.02 % EDTA and 0.25 % trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0251 Cell Name   SCH
LOT No.   04192019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.5 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   79.0 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   80.1 Additional information   
Cell No.   JCRB0251 Cell Name   SCH
LOT No.   10162023 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   0.833-1.12x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.88x10^6
Viability at cell freezing (%)   87.66 Antibiotics Used   free
Passage Number   p21 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11,12
D7S820:10,11
D16S539:9
VWA:17
TH01:6,7
AM:X,Y
TPOX:11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   87.24 Additional information   
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