JCRB0253 MKN28

Cell information

Important Notice(s)

JCRB0253 MKN28 is derived from MKN74.

The problem in the identity of MKN28 cell line

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0253 Cell Name   MKN28
Profile   Stop providing for the issue of quality control. (Please contact us for more information) STR-PCR analysis indicated the MKN28 is derived from MKN74. Other Name   MKN28
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   stomach
Case history    Metastasis   
Tissue Metastasized    Genetics   stomach, 70 year-old female.
Life Span    Crisis PDL   
Morphology    Character   stomach, 70 year-old female.
Classify    Established by   Hojo,H.
Registered by   Motoyama & Watanabe Regulation for Distribution   
Comment   Moderately differentiated tubular adenocarcinoma. Mycoplasma-eliminated cells by MC-210. Year   1989
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/10-1/20 Race   
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1416
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 908139
Reference
Pubmed id:2736210Production of transforming growth factor-alpha in human tumour cell lines.
Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K
Br J Cancer. 1989 May;59(5):761-5
Pubmed id:3095279Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel.
Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M
Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:3962675Comparison of seven cell lines derived from human gastric carcinomas.
Motoyama T,Hojo H,Watanabe H
Acta Pathol Jpn. 1986 Jan;36(1):65-83

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LOT Information

Cell No.   JCRB0253 Cell Name   
LOT No.   09012009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Cells were harvested aftert treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   1.0 x 10^7
Viability at cell freezing (%)   91.3 Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0253 Cell Name   MKN28
LOT No.   081495 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (JRH.12103-78) Temperature   37 C
Cell Density at Seeding   5.6x10^4 cells/ml Methods for Passages   Treat cells with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P18 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0253 Cell Name   MKN28
LOT No.   022289 Lot Specification   distribution
Medium   RPMI 1640 + FCS 10% Temperature   37
Cell Density at Seeding   1/10-1/20 Methods for Passages   0.01% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3x10^6
Viability at cell freezing (%)   85% Antibiotics Used   free
Passage Number   P14* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,G6PD,NP,PepB,AST,MD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0253 Cell Name   
LOT No.   022698 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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