JCRB0301 XP2OS(SV)
Cell information
Important Notice(s)Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0301 | Cell Name | XP2OS(SV) |
|---|---|---|---|
| Profile | xeroderma pigmentosum (A), SV40 transformed | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Xeroderma pigmentosum (A), SV40 transformed | |
| Life Span | Crisis PDL | ||
| Morphology | Character | Xeroderma pigmentosum (A), SV40 transformed | |
| Classify | Established by | Takebe,H. | |
| Registered by | Takebe,H. | Regulation for Distribution | |
| Comment | Hereditary disease with high cancer risk,xeroderma pigmentosum (autosomal recessive),complementation group A,SV40-transformed skin fibroblast. | Year | 1985 |
| Medium | alfa-MEM, 10% heat-inactivated fetal bovine serum. (establish: Eagle's minimal essential medium with 10% fetal calf serum ) | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.25% trypsin. |
| Cell Number on Passage | split ratio=1/5 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:4373650 | Comparative studies of host-cell reactivation, colony forming ability and excision repair after UV irradiation of xeroderma pigmentosum, normal human and some other mammalian cells. Takebe H,Nii S,Ishii MI,Utsumi H Mutat Res. 1974 Dec;25(3):383-90 |
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LOT Information
| Cell No. | JCRB0301 | Cell Name | XP2OS(SV) |
|---|---|---|---|
| LOT No. | 02152011 | Lot Specification | distribution |
| Medium | MEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 1.8-2.3x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.82x10^6 |
| Viability at cell freezing (%) | 99.3 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:9 D7S820:8,12 D16S539:12 VWA:14,16 TH01:9 AM:X TPOX:11 CSF1PO:10,11 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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