JCRB0305 XP2SA
Cell information
Important Notice(s)Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0305 | Cell Name | XP2SA |
|---|---|---|---|
| Profile | xeroderma pigmentosum (variant type), fibroblast | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 44 |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | karyotype=46,XX | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Xeroderma pigmentosum (Variant), fibroblast. Doubling time=24hr. |
| Classify | Established by | Ikenaga,M. | |
| Registered by | Ikenaga,M. | Regulation for Distribution | |
| Comment | Hereditary disease with high cancer risk,xeroderma pigmentosum (autosomal recessive), skin fibroblast. sister of XP1SA | Year | 1985 |
| Medium | RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: Dulbecco's modified Eagle's medium with 10% fetal calf serum.) | Methods for Passages | trypsin |
| Cell Number on Passage | split ratio=1/3 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | + | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:3814505 | Sensitivity to UV radiation of fibroblasts from a Japanese group A xeroderma pigmentosum patient with mild neurological abnormalities. Sato K,Watatani M,Ikenaga M,Kozuka T,Kitano Y,Yoshikawa K,Mimaki T,Abe J,Sugita T Br J Dermatol. 1987 Jan;116(1):101-8 |
| Pubmed id:7155047 | Kinetic analysis of polyethylene glycol-induced cell fusion in cultured human fibroblasts: its application to genetic complementation analysis of xeroderma pigmentosum. Sato K,Ikenaga M,Sano S Med J Osaka Univ. 1982 Sep;33(1-2):19-28 |
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LOT Information
| Cell No. | JCRB0305 | Cell Name | XP2SA |
|---|---|---|---|
| LOT No. | 03182011 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA027K03911,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 9.71-13.3x10^3cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/3) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.04x10^6 |
| Viability at cell freezing (%) | 97.5 | Antibiotics Used | free |
| Passage Number | p9 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:8,9 D7S820:11 D16S539:9 VWA:18 TH01:6 AM:X TPOX:11 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0305 | Cell Name | XP2SA |
|---|---|---|---|
| LOT No. | 02102017 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA12J396,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 1.2-2.1x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.3x10^6 |
| Viability at cell freezing (%) | 95.1 | Antibiotics Used | free |
| Passage Number | p13 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:8,9 D7S820:11 D16S539:9 VWA:18 TH01:6 AM:X TPOX:11 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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