JCRB0307 XPL15OS
Cell information
Important Notice(s)Cell type:human mutant cell repository from Kyoto University (View Pricing Information)
JCRB No. | JCRB0307 | Cell Name | XPL15OS |
---|---|---|---|
Profile | xeroderma pigmentosum (A), lymphoblastoid | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | Age | ||
Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | Xeroderma pigmentosum (A), lymphoblastoid | |
Life Span | Crisis PDL | ||
Morphology | Character | Xeroderma pigmentosum (A), lymphoblastoid | |
Classify | Established by | Furuyama,J. | |
Registered by | Tatsumi,K. | Regulation for Distribution | |
Comment | Hereditary disease with high cancer risk,xeroderma pigmentosum (autosomal recessive),complementation group A,EB virus-transformed lymphoblastoid cell line. | Year | 1985 |
Medium | RPMI1640 medium with 10% fetal calf serum. | Methods for Passages | 1/2.5 split (suspension culture) |
Cell Number on Passage | 4x10^5 cells/ml | Race | |
CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
---|---|---|---|---|---|---|---|---|---|
Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
---|---|
Pubmed id:2805228 | Predominance of Mex+ cells in newly-established human lymphoblastoid cell lines. Arita I,Tachibana A,Takebe H,Tatsumi K Carcinogenesis. 1989 Nov;10(11):2067-73 |
Images |
---|
LOT Information
Cell No. | JCRB0307 | Cell Name | XPL15OS |
---|---|---|---|
LOT No. | 05282010 | Lot Specification | distribution |
Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
Cell Density at Seeding | 2.5-6.4x10^5 cells/ml | Methods for Passages | dilution (split ratio=1/2.5) |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.0x10^6 |
Viability at cell freezing (%) | 97.1 | Antibiotics Used | free |
Passage Number | p5* | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | D5S818:12,13 D13S317:9,10 D7S820:12 D16S539:11,13 VWA:17,18 TH01:8,9 AM:X TPOX:8 CSF1PO:12 |
Adhesion | No | Exoteric Gene | NT |
Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
Viability immediately after thawing (%) | Additional information |
Images |
---|
Cell No. | JCRB0307 | Cell Name | XPL15OS |
---|---|---|---|
LOT No. | 971023 | Lot Specification | distribution |
Medium | RPMI1640 medium with 10% fetal calf serum. | Temperature | 37 C |
Cell Density at Seeding | 4 x 10^5 cells/ml | Methods for Passages | Simple dilution, split 1:2.5. |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | |
Viability at cell freezing (%) | Antibiotics Used | ||
Passage Number | PDL | ||
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | |
Adhesion | No | Exoteric Gene | NT |
Medium for Freezing | CO2 Conc. | ||
Viability immediately after thawing (%) | Additional information |