JCRB0311 RB16KY
Cell information
Important Notice(s)JCRB0311 RB16KY, JCRB0312 RB24KY: media used for cultivation
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0311 | Cell Name | RB16KY |
|---|---|---|---|
| Profile | fibroblast from retinoblastoma(13q-) patient | Other Name | RB16KY |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | retino blastoma | Metastasis | No |
| Tissue Metastasized | Genetics | Retinoblastoma(13q-), fibroblast | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Normal fibroblast from retinoblastoma patient. |
| Classify | normal | Established by | Sasaki,M. |
| Registered by | Sasaki,M. | Regulation for Distribution | |
| Comment | Patient with herediatry tumour,retinoblastoma due to germinal chromosome mutation del(13)(q14q22),bilateral tumours,skin fibroblast. | Year | 1985 |
| Medium | RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: alpha-MEM with 10% fetal calf serum) | Methods for Passages | trypin |
| Cell Number on Passage | split ratio=1/3 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:7057793 | Radiosensitivity of fibroblasts from patients with retinoblastoma and chromosome-13 anomalies. Ejima Y,Sasaki MS,Utsumi H,Kaneko A,Tanooka H Mutat Res. 1982 Feb;103(2):177-84 |
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LOT Information
| Cell No. | JCRB0311 | Cell Name | RB16KY |
|---|---|---|---|
| LOT No. | 06222011 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA027K03911,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 5.99-14.6x10^3cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.13x10^6 |
| Viability at cell freezing (%) | 97.4 | Antibiotics Used | free |
| Passage Number | p5 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:8,13 D7S820:10,11 D16S539:9,10 VWA:14,16 TH01:7 AM:X,Y TPOX:11 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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