JCRB0312 RB24KY
Cell information
Important Notice(s)JCRB0311 RB16KY, JCRB0312 RB24KY: media used for cultivation
Cell type:human mutant cell repository from Kyoto University (View Pricing Information)
JCRB No. | JCRB0312 | Cell Name | RB24KY |
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Profile | fibroblast from retinoblastoma (bilateral) patient | Other Name | RB24KY |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | Age | ||
Identity | available | Tissue for Primary Cancer | skin |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | Retinoblastoma (Bilateral), fibroblast | |
Life Span | Crisis PDL | ||
Morphology | Character | Retinoblastoma (Bilateral), fibroblast | |
Classify | Established by | Sasaki,M. | |
Registered by | Sasaki,M. | Regulation for Distribution | |
Comment | Patient with hereditary tumor,retinoblastoma (autosomal dominant),familial,bilateral tumors,skin fibroblast. | Year | 1985 |
Medium | RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: alpha-MEM with 10% fetal calf serum) | Methods for Passages | trypsin |
Cell Number on Passage | split ratio=1/3 | Race | |
CO2 Conc. | 5 % | Tissue Sampling | skin |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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EBV | - | HBV | - | ||||||
HHV6 | + | HTLV-1 | - | ||||||
HHV7 | - | HTLV-2 | - | ||||||
BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
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Pubmed id:7057793 | Radiosensitivity of fibroblasts from patients with retinoblastoma and chromosome-13 anomalies. Ejima Y,Sasaki MS,Utsumi H,Kaneko A,Tanooka H Mutat Res. 1982 Feb;103(2):177-84 |
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LOT Information
Cell No. | JCRB0312 | Cell Name | RB24KY |
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LOT No. | 07012011 | Lot Specification | distribution |
Medium | RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA027K03911,non HI) | Temperature | 37 C |
Cell Density at Seeding | 6.52-23.5x10^3cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.17x10^6 |
Viability at cell freezing (%) | 97.4 | Antibiotics Used | free |
Passage Number | p5 | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | D5S818:9,10 D13S317:8,15 D7S820:9,11 D16S539:9,13 VWA:18 TH01:6,9 AM:X,Y TPOX:8 CSF1PO:12 |
Adhesion | Yes | Exoteric Gene | NT |
Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
Viability immediately after thawing (%) | Additional information |
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