JCRB0317 BS2CH
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0317 | Cell Name | BS2CH |
|---|---|---|---|
| Profile | Bloom's syndrome, fibroblast | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Bloom's syndrome, fibroblast | |
| Life Span | Crisis PDL | ||
| Morphology | Character | Bloom's syndrome, fibroblast | |
| Classify | Established by | Sasaki,M. | |
| Registered by | Sasaki,M. | Regulation for Distribution | |
| Comment | Hereditary disease with high cancer risk,Bloom's syndrome (autosomal recessive),chromosomal breakage syndrome,skin fibroblast,high frequency of spontaneous SCE. | Year | 1985 |
| Medium | RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: alpha MEM medium with 10 % fetal calf serum.) | Methods for Passages | 0.25 % trypsin, split ratio = 1/4 |
| Cell Number on Passage | 1x10^5 cells/100mm dish | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB0317 | Cell Name | BS2CH |
|---|---|---|---|
| LOT No. | 06242010 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA027K03911,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 5-9x10^3 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and split ratio=1/4. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.18x10^6 |
| Viability at cell freezing (%) | 97.8 | Antibiotics Used | free |
| Passage Number | p9 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:8,10 D7S820:8,12 D16S539:10,11 VWA:16,19 TH01:7,9 AM:X TPOX:8,11 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0317 | Cell Name | BS2CH |
|---|---|---|---|
| LOT No. | 08292008 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 20% fetal bovine serum(Hyclone GRD0051,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 4.6x10^5 cells /dish | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and split ratio=1/4 |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.2x10^6 |
| Viability at cell freezing (%) | 99.0 | Antibiotics Used | free |
| Passage Number | p9 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:8,10 D7S820:8,12 D16S539:10,11 VWA:16,19 TH01:7,9 AM:X TPOX:8,11 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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