JCRB0324 BCNS1KO
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0324 | Cell Name | BCNS1KO |
|---|---|---|---|
| Profile | basal cell nevus syndrome, fibroblasts | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Basal cell nevus syndrome, fibroblasts | |
| Life Span | Crisis PDL | ||
| Morphology | Character | Basal cell nevus syndrome, fibroblasts | |
| Classify | Established by | Fujiwara,Y. | |
| Registered by | Sasaki,M. | Regulation for Distribution | |
| Comment | Patient with hereditary tumor,Basal cell nevus syndrome,nevoid basal cell carcinoma (autosomal dominant),skin fibroblast. | Year | 1986 |
| Medium | RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: Eagle's minimal essential medium with non essential amino acids + 10 %-15 % FBS) | Methods for Passages | 0.25 % trypsin |
| Cell Number on Passage | split ratio=1/2-1/4 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB0324 | Cell Name | BCNS1KO |
|---|---|---|---|
| LOT No. | 10202008 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% fetal bovine serum(Hyclone GRD0051,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 6.0x10^5 cells/100m/mdish | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and split 1/2-1/4. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.4x10^6 |
| Viability at cell freezing (%) | 99.6 | Antibiotics Used | free |
| Passage Number | p14 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:8,9 D7S820:10,12 D16S539:9,11 VWA:14,17 TH01:6 AM:X,Y TPOX:8,11 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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