JCRB0327 XP40OS

Cell information

Important Notice(s)

XP（色素性乾皮症患者）由来細胞

Cell type：human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB0327 Cell Name   XP40OS
Profile   xeroderma pigmentosum (C), fibroblast Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   Xeroderma pigmentosum (C), fibroblast
Life Span   infinite Crisis PDL   
Morphology    Character   Xeroderma pigmentosum (C), fibroblast
Classify    Established by   Ikenaga,M.
Registered by   Sasaki,M. Regulation for Distribution   
Comment   Group C XP cells. Year   1988
Medium   RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: alpha-MEM medium with 20% fetal calf serum) Methods for Passages   Replate every 7 days. Remove cells by Trypsin-EDTA and dilute to 1:2.
Cell Number on Passage   2-5x10^6 cells/100mm dish Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3250
Reference
Pubmed id:7155047Kinetic analysis of polyethylene glycol-induced cell fusion in cultured human fibroblasts: its application to genetic complementation analysis of xeroderma pigmentosum.
Sato K,Ikenaga M,Sano S
Med J Osaka Univ. 1982 Sep;33(1-2):19-28


Research results by users. Click！
Pubmed id:26697316 Whole-exome sequencing of fibroblast and its iPS cell lines derived from a patient diagnosed with xeroderma pigmentosum.
Okamura K,Toyoda M,Hata K,Nakabayashi K,Umezawa A
Genom Data. 2015 Dec;6():4-6

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LOT Information

Cell No.   JCRB0327 Cell Name   XP40OS
LOT No.   05122011 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA027K03911,non HI) Temperature   37 C
Cell Density at Seeding   0.40-2.7x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   p16 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13,14
D13S317:8,9
D7S820:10,12
D16S539:10,12
VWA:17,18
TH01:9
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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