JCRB0330 RBL182T
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0330 | Cell Name | RBL182T |
|---|---|---|---|
| Profile | lymphoblastoid, from patient with hereditary disease with high cancer risk retinoblastoma (chromosome 13-deletion) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | lymphoblastoid | |
| Life Span | Crisis PDL | ||
| Morphology | Character | lymphoblastoid | |
| Classify | Established by | Sasaki,M. | |
| Registered by | Sasaki,M. | Regulation for Distribution | |
| Comment | hereditary disease with high cancer risk retinoblastoma (chromosome 13-deletion) | Year | 1990 |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | 1/2.5 split (suspension culture) |
| Cell Number on Passage | 4x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB0330 | Cell Name | RBL182T |
|---|---|---|---|
| LOT No. | 06102010 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 3.3-7.8x10^5 cells/ml | Methods for Passages | dilution (split ratio=1/2.5) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 9.1x10^6 |
| Viability at cell freezing (%) | 97.8 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:9 D7S820:12 D16S539:11 VWA:14,15 TH01:7 AM:X,Y TPOX:8,9 CSF1PO:12,13 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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