JCRB0332 AT(L)6KY
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0332 | Cell Name | AT(L)6KY |
|---|---|---|---|
| Profile | EBV transformed B lymphoblastoid cell line, ataxia telangiectasia | Other Name | AT(L)6KY |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 8 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | ataxia telangiectasia | Metastasis | No |
| Tissue Metastasized | Genetics | 2n=46,46,XY | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | EBV transformed B cell lymphoblastoid cell line |
| Classify | transformed | Established by | Ejima,Y. |
| Registered by | Ejima,Y. | Regulation for Distribution | |
| Comment | Year | 1991 | |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | suspension culture, dilution 1/2.5 when subculture |
| Cell Number on Passage | 4x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | + | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB0332 | Cell Name | AT(L)6KY |
|---|---|---|---|
| LOT No. | 07132006 | Lot Specification | distribution |
| Medium | RPMI1640 with 10% heat inactivated fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 4.8x10^4 cells/sq.cm | Methods for Passages | suspension culture,dilution 1/2.5 when subculture |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.5x10^6 |
| Viability at cell freezing (%) | 96.4 | Antibiotics Used | Free |
| Passage Number | p6* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:11,12 D7S820:10,11 D16S539:11 VWA:14,18 TH01:7,9 AM:X,Y TPOX:8,9 CSF1PO:11,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker:BLC-1 | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0332 | Cell Name | |
|---|---|---|---|
| LOT No. | 03-07-11 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal calf serum. Please be careful to pH because the RPMI 1640 tends to become alkaline pH, which is harmful for the cells. | Temperature | 37 C |
| Cell Density at Seeding | 4 x 10^5 cells/ml | Methods for Passages | Suspension culture, dilution 1/2.5 when subcultivation. |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | Culture medium with 10% DMSO | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |