-Detailed Information [JCRB0427]-

JCRB0427 HuO9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0427	Cell Name	HuO9
Profile	A cell line established from a human osteosarcoma transplanted into athymic nude mice after fifth passages. The cell line expressed 1,25(OH)2D3-responsive alikaline phosphatase and produced tumors in the dorsum of nude mice.	Other Name	HuO9
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	13
Identity	available	Tissue for Primary Cancer	sarcoma
Case history	osteosarcoma	Metastasis
Tissue Metastasized		Genetics	Liver/bone/kidney-type alkaline phosphatase.
Life Span	infinite	Crisis PDL
Morphology	fibroblast-like	Character	Secretes osteocalcin & responds to vitamin D3.
Classify	tumor	Established by	Kawai,A.et al.
Registered by	Namba,M.	Regulation for Distribution	No commercial purpose.
Comment		Year
Medium	RPMI1640 medium with 10% fetal bovine serum.	Methods for Passages	Cells treated with 0.2 % trypsin and 0.02% EDTA.
Cell Number on Passage	1-4 x 10^5 cells/ml	Race	Jpanese
CO2 Conc.	5 %	Tissue Sampling	bone (right femur)
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1298
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	907072

Reference
Pubmed id:2170070	A newly established human osteosarcoma cell line with osteoblastic properties. Kawai A Clin Orthop Relat Res. 1990 Oct;(259):256-67
Pubmed id:2691514	Two distinct cell lines derived from a human osteosarcoma. Kawai A,Ozaki T,Ikeda S,Oda T,Miyazaki M,Sato J,Taketa K,Inoue H J Cancer Res Clin Oncol. 1989;115(6):531-6

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LOT Information

081291
04082014
07272020

Cell No.	JCRB0427	Cell Name	HuO9
LOT No.	081291	Lot Specification	distribution
Medium	RPMI1640 + 0.2% lactalbmin hydrolysate + 10% FBS	Temperature	37 C
Cell Density at Seeding	1-4x10^5	Methods for Passages	0.2 % trypsin treatment
Doubling Time	doubling time=5	Cell Number in Vial (cells/1ml)	>10^6 /ml
Viability at cell freezing (%)		Antibiotics Used	kanamycin (60ug/ml)
Passage Number	>20	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	not done
Chromosome Mode		Chromosome Information	The cells widely distribute in a triploid range.
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0427	Cell Name	HuO9
LOT No.	04082014	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	3.4 - 7.8 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time		Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	92.3	Antibiotics Used	free
Passage Number	P30	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:13 D13S317:11 D7S820:10 D16S539:11 VWA:14 TH01:9 AM:X TPOX:9 CSF1PO:10,11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	92.3	Additional information

Cell No.	JCRB0427	Cell Name	HuO9
LOT No.	07272020	Lot Specification	distribution
Medium	RPMI1640 medium(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396)	Temperature	37 C
Cell Density at Seeding	1.7-2.4x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(1/3 split)
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.4x10^6
Viability at cell freezing (%)	92.30	Antibiotics Used	free
Passage Number	p31	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:13 D13S317:11 D7S820:10 D16S539:11 VWA:14 TH01:9 AM:X TPOX:9 CSF1PO:10,11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	50.3	Additional information	We recommend to start culture with 25T bottle or 60mm dish,because viability after thawing is about 50%.

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