JCRB0428 HuO9N2
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0428 | Cell Name | HuO9N2 |
|---|---|---|---|
| Profile | osteosarcoma | Other Name | O9N2 |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 13 |
| Identity | available | Tissue for Primary Cancer | sarcoma |
| Case history | osteosarcoma | Metastasis | |
| Tissue Metastasized | Genetics | Vitamin D3 responsive ALP production. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Polygonal shape | Character | Tumorigenic cell line. Growth rate is very slow. |
| Classify | tumor | Established by | Kawai,A. |
| Registered by | Namba,M. | Regulation for Distribution | No commercial purpose. |
| Comment | Year | 1991 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.2 % trypsin. |
| Cell Number on Passage | More than 1.5x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | bone |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2170070 | A newly established human osteosarcoma cell line with osteoblastic properties. Kawai A Clin Orthop Relat Res. 1990 Oct;(259):256-67 |
| Pubmed id:2691514 | Two distinct cell lines derived from a human osteosarcoma. Kawai A,Ozaki T,Ikeda S,Oda T,Miyazaki M,Sato J,Taketa K,Inoue H J Cancer Res Clin Oncol. 1989;115(6):531-6 |
| Images |
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LOT Information
| Cell No. | JCRB0428 | Cell Name | HuO9N2 |
|---|---|---|---|
| LOT No. | 081291 | Lot Specification | distribution |
| Medium | RPMI1640 + 0.2% lactalbumin hydrolysate + 10% FBS | Temperature | 37 C |
| Cell Density at Seeding | >1.5x10^5 | Methods for Passages | 0.2 % trypsin |
| Doubling Time | doubling time=1 | Cell Number in Vial (cells/1ml) | >10^6 /ml |
| Viability at cell freezing (%) | Antibiotics Used | kanamycin (60ug/ml) | |
| Passage Number | >24 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | not done |
| Chromosome Mode | Chromosome Information | No. of chr. distributed in a triploid range. | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0428 | Cell Name | |
|---|---|---|---|
| LOT No. | 031297 | Lot Specification | distribution |
| Medium | RPMI1640 with 10% FBS (heat-inactivation is recom-mended) (FBS lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 5-9 x 10^4 cells/ml | Methods for Passages | 0.2% trypsin and 0.02% EDTA |
| Doubling Time | D.T.=ca 5 days | Cell Number in Vial (cells/1ml) | 7.1 x 10^5 |
| Viability at cell freezing (%) | 82.0 | Antibiotics Used | free |
| Passage Number | P15* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |