JCRB0430 KMS-12-PE
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0430 | Cell Name | KMS-12-PE |
|---|---|---|---|
| Profile | myeloma, late stage of B-cell differentiation | Other Name | KMS-12_PE |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 64 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | myeloma | Metastasis | |
| Tissue Metastasized | Genetics | late stage B-cell differentiation, EBV Ag.negative | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | non-producing, transferrin receptors positive |
| Classify | transformed | Established by | Namba,M. et al. |
| Registered by | Namba,M. | Regulation for Distribution | No commercial purpose. |
| Comment | Year | ||
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | Simple dilution. |
| Cell Number on Passage | 5x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | pleural effusion |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2768132 | Establishment of five human myeloma cell lines. Namba M,Ohtsuki T,Mori M,Togawa A,Wada H,Sugihara T,Yawata Y,Kimoto T In Vitro Cell Dev Biol. 1989 Aug;25(8):723-9 |
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LOT Information
| Cell No. | JCRB0430 | Cell Name | |
|---|---|---|---|
| LOT No. | 102396 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% FCS (heat-inactivation isrecommended) (FCS lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3.1 x 10^6 | |
| Viability at cell freezing (%) | 72.7 | Antibiotics Used | free |
| Passage Number | P6* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | confirmed as human by NP, G6PD (type B), MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0430 | Cell Name | KMS-12-PE |
|---|---|---|---|
| LOT No. | 05012015 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat.# 10091). | Temperature | 37 C |
| Cell Density at Seeding | 1 - 2 x 10^5 cells/ml | Methods for Passages | Simple dilution (suspension culture). |
| Doubling Time | Cell Number in Vial (cells/1ml) | 8.4 x 10^5 | |
| Viability at cell freezing (%) | 70 | Antibiotics Used | free |
| Passage Number | Unknown (12 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 70 | Additional information |
| Cell No. | JCRB0430 | Cell Name | KMS-12-PE |
|---|---|---|---|
| LOT No. | 10252022 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) | Temperature | 37 C |
| Cell Density at Seeding | 3.9 - 4.9 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). |
| Doubling Time | 37 - 67 hrs. | Cell Number in Vial (cells/1ml) | 2.5 x 10^6 |
| Viability at cell freezing (%) | 80.5 | Antibiotics Used | free |
| Passage Number | Unknown (7 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:14 D13S317:8 D7S820:11,12 D16S539:11 VWA:14,16 TH01:6,9 AM:X TPOX:8,11 CSF1PO:9 |
|
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 5% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 75.6 | Additional information |