JCRB0624 HSC-4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0624 Cell Name   HSC-4
Profile   Human squamous carcinoma cell line from tongue. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64
Identity   available Tissue for Primary Cancer   oral
Case history   cancer in tongue. Metastasis   
Tissue Metastasized    Genetics   squamous carcinoma, tongue, age 64, (M)
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   squamous carcinoma
Classify   tumor Established by   Momose,F.
Registered by   Momose,F. Regulation for Distribution   no
Comment    Year   
Medium   Eagle's minimal essential medium with 10% calf serum. Methods for Passages   Cells are treated with trypsin and EDTA. Cells are diluted 1/8 once a week for the subculture.
Cell Number on Passage   1 x 10^5 cells/10cm dish Race   Japanese
CO2 Conc.   5 % Tissue Sampling   tongue
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1289
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907062
Reference
Pubmed id:2228902Growth of the malignant and nonmalignant human squamous cells in a protein-free defined medium.
Rikimaru K,Toda H,Tachikawa N,Kamata N,Enomoto S
In Vitro Cell Dev Biol. 1990 Sep;26(9):849-56
Pubmed id:2164440Potent growth inhibition of human tumor cells in culture by arginine deiminase purified from a culture medium of a Mycoplasma-infected cell line.
Miyazaki K,Takaku H,Umeda M,Fujita T,Huang WD,Kimura T,Yamashita J,Horio T
Cancer Res. 1990 Aug 1;50(15):4522-7
Pubmed id:2585303Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas.
Momose F,Araida T,Negishi A,Ichijo H,Shioda S,Sasaki S
J Oral Pathol Med. 1989 Aug;18(7):391-5

Images



LOT Information

Cell No.   JCRB0624 Cell Name   
LOT No.   06292012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   2.5 - 6.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0624 Cell Name   
LOT No.   04012002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   4-6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   D.T. = ca 25 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P9* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0624 Cell Name   HSC-4
LOT No.   112696 Lot Specification   Distribution
Medium   Eagle's minimal essential medium with 10% calf serum (C.C.Labs.10910-01). Temperature   37 C
Cell Density at Seeding   1-1.5x10^5 cells/ml Methods for Passages   Wash cells with PBS(-) and treat them with 0.02% EDTA and 0.25% trypsin for 10 min at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP, LDH examined. (human).
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0624 Cell Name   HSC-4
LOT No.   091985 Lot Specification   distribution
Medium   MEM + 10%CS Temperature   37
Cell Density at Seeding   1 X 10^5 Methods for Passages   trypsin-EDTA, 1:8 dilution once a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0624 Cell Name   
LOT No.   940606 Lot Specification   distribution
Medium   MEM + 10%CS Temperature   37
Cell Density at Seeding    Methods for Passages   trypsin-EDTA, 1:8 dilution once a week
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   kanamycin 60 ug/ml
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0624 Cell Name   HSC-4
LOT No.   03202018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (NCS; Biowest Cat. # S0750). Temperature   37 C
Cell Density at Seeding   1.5 - 4.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treaatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   9.5 x 10^5
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
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