JCRB0740 NG-3Y1-T308

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0740 Cell Name   NG-3Y1-T308
Profile   3Y1 derivative, transformed by MNNG, (Fischer rat) Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex   F Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   whole embryo,transformed 3Y1 by MNNG treatment
Life Span   infinite Crisis PDL   
Morphology    Character   whole embryo,transformed 3Y1 by MNNG treatment
Classify   transformed Established by   Kimura,G.
Registered by   Kimura,G. Regulation for Distribution   
Comment    Year   1988
Medium   Dulbecco's modified Eagle's medium (0.1% glucose) with 10 % fetal calf serum Methods for Passages   0.05% trypsin + 5mM EDTA. Dilute 1/10-1/20. Prepare many frozen ampules when received. Use new stock
Cell Number on Passage   Less than 1x10^6 cells/50mm di Race   
CO2 Conc.   5 % Tissue Sampling   embryo/fetus whole body
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_H228
Reference
Pubmed id:3447927Maintenance of postconfluence stationary cell density by transient increase and decrease in cell number upon medium renewals in rat 3Y1 fibroblasts. Diminution of the decrease in cell number after cell transformation by N-methyl-N'-nitro-N-nitrosoguanidine.
Ohno K,Zaitsu H,Kimura G
Fukuoka Igaku Zasshi. 1987 Dec;78(12):569-77
Pubmed id:6207637Polyester wax embedding and sectioning technique for immunohistochemistry.
Kusakabe M,Sakakura T,Nishizuka Y,Sano M,Matsukage A
Stain Technol. 1984 May;59(3):127-32

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LOT Information

Cell No.   JCRB0740 Cell Name   
LOT No.   091099 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   3-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca 33 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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