JCRB0745 T-23 clone 8

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0745 Cell Name   T-23 clone 8
Profile   normal, applicable for the mutation assay system in vitro Other Name   T-238
Animal   tupaia Strain   
Genus   Tupaia Species   belangeri
Sex   M Age   young
Identity    Tissue for Primary Cancer   lung
Case history   normal Metastasis   No
Tissue Metastasized    Genetics   normal
Life Span   finite Crisis PDL   
Morphology   epithelial-like Character   Applicable for the mutation assay system in vitro.
Classify   normal Established by   Inui,N. & Taketomi,M.
Registered by   Umeda,M. Regulation for Distribution   Not applied.
Comment    Year   1991
Medium    Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5596
Reference
Pubmed id:3331674Establishment of lung fibroblastic cell lines from a non-human primate Tupaia belangeri and their use in a forward gene mutation assay at the hypoxanthine-guanine phosphoribosyl transferase locus.
Taketomi M,Nishi Y,Ohkawa Y,Inui N
Mutagenesis. 1986 Sep;1(5):359-65

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LOT Information

Cell No.   JCRB0745 Cell Name   
LOT No.   02142013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.8 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   22 - 37 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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