JCRB0822 NUGC-3 Cell information
Cell type:general cells
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JCRB No.
JCRB0822
Cell Name
NUGC-3
Profile
adenocarcinoma, poorly differentiated
Other Name
Animal
human
Strain
Genus
Homo
Species
sapiens
Sex
M
Age
72
Identity
available
Tissue for Primary Cancer
stomach
Case history
Metastasis
Tissue Metastasized
Genetics
gastric cancer
Life Span
Crisis PDL
Morphology
Character
gastric cancer
Classify
Established by
Akiyama,S.
Registered by
Akiyama,M.
Regulation for Distribution
Comment
Year
1987
Medium
RPMI1640 medium with 10% fetal calf serum.
Methods for Passages
Cells are treated with 0.25% trypsin and dilute into fresh medium. Change medium twice a week.
Cell Number on Passage
split ratio=1/6
Race
CO2 Conc.
5 %
Tissue Sampling
stomach
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
tested
Detected RNA Virus
tested
CMV
-
parvoB19
-
HCV
-
HTLV-1
-
EBV
-
HBV
-
HIV-1
-
HTLV-2
-
HHV6
-
HTLV-1
-
HIV-2
-
HAV
-
HHV7
-
HTLV-2
-
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV
-
HIV-1
-
JCV
-
HIV-2
-
ADV
-
HPV18
-
Notes
Reference
Pubmed id:2121682 Alloimmunization with cultured human stomach cancer cell lines and the establishment of human-human hybridomas producing monoclonal antibodies. Koyama K,Akiyama K,Kawahara H,Egashira A,Murakami H Jpn J Cancer Res. 1990 Oct;81(10):967-70
Pubmed id:3172586 Characteristics of three human gastric cancer cell lines, NU-GC-2, NU-GC-3 and NU-GC-4. Akiyama S,Amo H,Watanabe T,Matsuyama M,Sakamoto J,Imaizumi M,Ichihashi H,Kondo T,Takagi H Jpn J Surg. 1988 Jul;18(4):438-46
Movies
LOT Information
Cell No.
JCRB0822
Cell Name
LOT No.
11042011
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099)
Temperature
37 C
Cell Density at Seeding
approx. 1 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time
approx. 32 hrs.
Cell Number in Vial (cells/1ml)
2.2 x 10^6
Viability at cell freezing (%)
98.2
Antibiotics Used
free
Passage Number
P38
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI 1640
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0822
Cell Name
LOT No.
04272009
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099)
Temperature
37 C
Cell Density at Seeding
1 - 2 x 10^5 cells/ml
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
28 - 45 hrs.
Cell Number in Vial (cells/1ml)
3.6 x 10^6
Viability at cell freezing (%)
97.8
Antibiotics Used
free
Passage Number
P39
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI 1640
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0822
Cell Name
LOT No.
06062005
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S)
Temperature
37 C
Cell Density at Seeding
0.5 - 1.5 x 10^5 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA
Doubling Time
approx. 26 hrs
Cell Number in Vial (cells/1ml)
3.3 x 10^6
Viability at cell freezing (%)
96.2
Antibiotics Used
free
Passage Number
P38
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as human by human by NP, G6PD (type B), AST.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI 1640
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0822
Cell Name
NUGC-3
LOT No.
120396
Lot Specification
distribution
Medium
RPMI1640 medium with 10% heat inactivated fetal calf serum (Mitsubishi Kagaku, PVF01)
Temperature
37 C
Cell Density at Seeding
1-1.5x10^5 cells/ml
Methods for Passages
Wash cells with PBS(-) and treat them with 0.02% EDTA and 0.25% trypsin for 10 min at 37 C.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
1.2x10^6
Viability at cell freezing (%)
97.0
Antibiotics Used
free
Passage Number
P37
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
Culture medium with 5% DMSO.
CO2 Conc.
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0822
Cell Name
LOT No.
090195
Lot Specification
distribution
Medium
RPMI1640 + 10% FCS
Temperature
37
Cell Density at Seeding
Methods for Passages
0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time
Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)
Antibiotics Used
Passage Number
PDL
Sterility: MYCOPLASMA
Sterility: BACTERIA
Sterility: FUNGI
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB0822
Cell Name
NUGC-3
LOT No.
02262016
Lot Specification
distribution
Medium
RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091).
Temperature
Cell Density at Seeding
0.8 - 1.4 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 28 hrs.
Cell Number in Vial (cells/1ml)
3.0 x 10^6
Viability at cell freezing (%)
91
Antibiotics Used
free
Passage Number
P38
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI-1640
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
91
Additional information
Cell No.
JCRB0822
Cell Name
NUGC-3
LOT No.
11162017
Lot Specification
distribution
Medium
RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012).
Temperature
37 C
Cell Density at Seeding
0.9 - 1.7 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 1 day
Cell Number in Vial (cells/1ml)
2.2 x 10^6
Viability at cell freezing (%)
96.5
Antibiotics Used
free
Passage Number
P38
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI1640
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
96.5
Additional information
Lot# is mislabeled as 11062017.
Cell No.
JCRB0822
Cell Name
NUGC-3
LOT No.
11072019
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012)
Temperature
37 C
Cell Density at Seeding
4.0 - 8.2 x 10^4 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 32 hrs.
Cell Number in Vial (cells/1ml)
1.2 x 10^6
Viability at cell freezing (%)
99
Antibiotics Used
free
Passage Number
P38
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI1640
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
98
Additional information
Cell No.
JCRB0822
Cell Name
NUGC-3
LOT No.
01172022
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500)
Temperature
37 C
Cell Density at Seeding
0.9 - 1.2 x 10^5
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
32 - 56 hrs
Cell Number in Vial (cells/1ml)
3.9 x 10^6
Viability at cell freezing (%)
89.2
Antibiotics Used
free
Passage Number
P38
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI1640
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
89.5
Additional information
Cell No.
JCRB0822
Cell Name
NUGC-3
LOT No.
08042023
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437. Lot 1072322)
Temperature
37C
Cell Density at Seeding
0.7 - 1.2 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 35 - 40 hrs.
Cell Number in Vial (cells/1ml)
1.9 x 10^6
Viability at cell freezing (%)
95.6
Antibiotics Used
free
Passage Number
P37
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
5% DMSO, 20% FBS - RPMI1640
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
97.0
Additional information