-Detailed Information [JCRB0831]-

JCRB0831 HO-1-N-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0831	Cell Name	HO-1-N-1
Profile	squamous carcinoma, buccal mucosa	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	oral
Case history		Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	squamous cell carcinoma
Classify		Established by
Registered by		Regulation for Distribution
Comment		Year
Medium	Dulbecco's modified Eagle's medium - F12 mdeium (1:1 mix) with 10% fetal calf serum.	Methods for Passages	Cells are treated with 0.1% trypsin and 0.02% EDTA and dilute in fresh medium.
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	buccal mucosa
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1284
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	924111

Images

LOT Information

04092001
02292001
090495
08212015
10232019

Cell No.	JCRB0831	Cell Name
LOT No.	04092001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium : Ham's F12 medium = 1:1 with 10% fetal calf serum	Temperature	37 C
Cell Density at Seeding	1 x 10^5 cells/ml	Methods for Passages	Hardly detach. Wash 3 times with PBS(-) and treat cells with 0.25% trypsin and 0.02% EDTA in PBS(-) for 5-10 min at 37 C.
Doubling Time		Cell Number in Vial (cells/1ml)	7.8 x 10^5
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	P45	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0831	Cell Name
LOT No.	02292001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium : Ham's F12 medium = 1:1 with 10% fetal calf serum	Temperature	37 C
Cell Density at Seeding	3-9 x 10^4 cells/ml	Methods for Passages	Hardly detach. Wash 3 times with PBS(-) and treat cells with 0.25% trypsin and 0.02% EDTA in PBS(-) for 5-10 min at 37C.
Doubling Time		Cell Number in Vial (cells/1ml)	6.0 x 10^5
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	P39	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0831	Cell Name
LOT No.	090495	Lot Specification	distribution
Medium	Dulbecco's MEM : Ham's F12 = 1:1 mixture with 10% fetal bovine serum.	Temperature	37
Cell Density at Seeding		Methods for Passages	monolayer culture
Doubling Time		Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)		Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0831	Cell Name	HO-1-N-1
LOT No.	08212015	Lot Specification	distribution
Medium	1:1 mixture of Dulbecco's modified Eagle's medium - Ham's F12 mdeium (DMEM/F12) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091).	Temperature	37 C
Cell Density at Seeding	6.9 - 8.7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 31 hrs	Cell Number in Vial (cells/1ml)	2.4 x 10^6
Viability at cell freezing (%)	97.9	Antibiotics Used	free
Passage Number	P38	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:9 D13S317:10,11 D7S820:10,13 D16S539:9,11 VWA:16,18 TH01:9 AM:X,Y TPOX:11 CSF1PO:12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM/F12	CO2 Conc.	5%
Viability immediately after thawing (%)	97.9	Additional information

Cell No.	JCRB0831	Cell Name	HO-1-N-1
LOT No.	10232019	Lot Specification	distribution
Medium	1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	2.8 - 5.2 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 31 hrs.	Cell Number in Vial (cells/1ml)	2.3 x 10^6
Viability at cell freezing (%)	97.1	Antibiotics Used	free
Passage Number	P38	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM/F12	CO2 Conc.	5%
Viability immediately after thawing (%)	97	Additional information