JCRB1009 RERF-GC-1B
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1009 | Cell Name | RERF-GC-1B |
|---|---|---|---|
| Profile | Human gastric cancer cell line established from a metastatic tissue of pyloric lymph nodes. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 41 |
| Identity | available | Tissue for Primary Cancer | stomach |
| Case history | gastric cancer | Metastasis | Yes |
| Tissue Metastasized | pyloric lymph nodes | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Gastric adenocarcinoma cell line established from metastatic tumor tissue of pyloric lymph nodes. |
| Classify | tumor | Established by | Kyoizumi,S. |
| Registered by | Kyoizumi,S. | Regulation for Distribution | |
| Comment | No reference informed when deposited. | Year | 2001 |
| Medium | RPMI1640 medium with 10% heat inactivated fetal calf serum. | Methods for Passages | Cells are harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 min. Subculture once two weeks. |
| Cell Number on Passage | split ratio = 1/4 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | stomach |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1009 | Cell Name | RERF-GC-1B |
|---|---|---|---|
| LOT No. | 10292008 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) | Temperature | 37 C |
| Cell Density at Seeding | approx. 1 x 10^5 cells/mL | Methods for Passages | 0.05% trypsin and 0.02% EDTA. Passage approx. once a week. |
| Doubling Time | approx. 2 days | Cell Number in Vial (cells/1ml) | 3.5 x 10^5 |
| Viability at cell freezing (%) | 94.2 | Antibiotics Used | free |
| Passage Number | P23 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information | Lot no. is labeled as 20081029. |
| Cell No. | JCRB1009 | Cell Name | RERF-GC-1B |
|---|---|---|---|
| LOT No. | 07192016 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). | Temperature | 37 C |
| Cell Density at Seeding | 3.3 x 4.2 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA. |
| Doubling Time | 49 hrs | Cell Number in Vial (cells/1ml) | 1.2 x 10^6 |
| Viability at cell freezing (%) | 95 | Antibiotics Used | free |
| Passage Number | P21 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 95 | Additional information | The cell name is mislabeled on vials. The correct cell name is RERF-GC-1B. |
| Cell No. | JCRB1009 | Cell Name | RERF-GC-1B |
|---|---|---|---|
| LOT No. | 06092022 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) | Temperature | 37 C |
| Cell Density at Seeding | 4.1 - 5.6 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 3 days | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | P22 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:10,12 D13S317:12 D7S820:10,13 D16S539:11 VWA:14,18 TH01:9 AM:X TPOX:11 CSF1PO:12,13 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94 | Additional information |