JCRB1018 HMY-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1018 Cell Name   HMY-1
Profile   Mlignant melanoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   62
Identity   available Tissue for Primary Cancer   melanoma
Case history   Melanoma occurred on the right sole. Metastasis   Yes
Tissue Metastasized   lymph node Genetics   Modal number of chromosome is 66.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Melanoma cell line established from metastasised lymph node. Pigments were observed at early stages but faded later when established as a cell line.
Classify   tumor Established by   Kondo,S. et.al.
Registered by   Hozumi,Y. Regulation for Distribution   
Comment    Year   2001
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.02% EDTA and 0.05% Trypsin for 3 minuts.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2950
Reference
Pubmed id:6239887Establishment of cell lines from human malignant melanomas and their application to the assessment of natural killer-like cytotoxicity.
Kondo S,Sato N,Sato K,Hozumi Y,Aso K
J Dermatol. 1984 Aug;11(4):328-34

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LOT Information

Cell No.   JCRB1018 Cell Name   
LOT No.   05012007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   1-1.5 x 10^5 cells/ml Methods for Passages   0.05% trypsin and 0.02% EDTA for 3 min at room temperature.
Doubling Time   40 hrs Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   P91 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1018 Cell Name   HMY-1
LOT No.   12062002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2.1 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   P88 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1018 Cell Name   HMY-1
LOT No.   08122020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.2 - 2.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time   approx. 37 hrs. Cell Number in Vial (cells/1ml)   6.5 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   P91 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   88.8 Additional information   
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