JCRB1021 RERF-LC-Ad2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1021 Cell Name   RERF-LC-Ad2
Profile   Human cancer cell line, adenocarcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer, adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   human lung cancer, adenocarcinoma cell line.
Classify   tumor Established by   Kyoizumi,S.
Registered by   Kyoizumi,S. Regulation for Distribution   
Comment    Year   2001
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 minutes. Subculture once a week.
Cell Number on Passage   split ration = 1/5 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1652
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LOT Information

Cell No.   JCRB1021 Cell Name   RERF-LC-Ad2
LOT No.   07172015 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.80-2.43x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.31x10^6
Viability at cell freezing (%)   99.3 Antibiotics Used   free
Passage Number   p18 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:8,12
D7S820:11
D16S539:11
VWA:16
TH01:6,9
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)   96 Additional information   
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Cell No.   JCRB1021 Cell Name   RERF-LC-Ad2
LOT No.   10012004 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:8,12
D7S820:11
D16S539:11
VWA:16
TH01:6,9
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1021 Cell Name   RERF-LC-Ad2
LOT No.   07022019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA at 37 C for 10 min. Passage interval will be 1 week.
Doubling Time   approx. 2.4 days Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90.1 Additional information   In the original description, 0.05% trypsin was used for passage. However the cells were hard to detach with 0.05% trypsin. In this lot, 0.25% trypsin was used.
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