JCRB1022 OUMS-23

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1022 Cell Name   OUMS-23
Profile   A human colon carcinoma cell line established from metastatic pericardial fluid. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   colon
Case history   familial adenomatous polyposis patient with colon cancer Metastasis   yes
Tissue Metastasized   pericardial fluid Genetics   Expression of the APC gene decreased to one-half compared with the normal fibroblast cells. No mutaion at codons 12 and 61 of H-, K-, and N-ras proto oncogenes.
Life Span    Crisis PDL   
Morphology   epithelial-like Character   Cells express heat-stable placental-type APase and carcinoembryonic antigen. Chromosomes are between 53 and 118. Cells can grow in soft agar at cloning efficiency of 6.5%. The cells form tumors in the nude mice diagnosed as differentiated adenocarcinoma.
Classify   tumor Established by   Miyazaki,M. & Namba,M.
Registered by   Namba,M. Regulation for Distribution   
Comment   Mycoplasmas decontaminated in the JCRB Cell Bank. Year   2002
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   Cells were treated with 0.1% trypsin and 0.02% EDTA when harvesting cells.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3088
Reference
Pubmed id:8576285Establishment and characterization of a human colon cancer cell line, OUMS-23, from a patient with familial adenomatous polyposis.
Miyazaki M,Tsuboi S,Mihara K,Kosaka T,Fukaya K,Kino K,Mori M,Namba M
J Cancer Res Clin Oncol. 1996;122(2):95-101

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LOT Information

Cell No.   JCRB1022 Cell Name   OUMS-23
LOT No.   01262009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   2.4 - 5.6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   1 x 10^6 (viable)
Viability at cell freezing (%)   60 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:12
D7S820:10,13
D16S539:11
VWA:16
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1022 Cell Name   OUMS-23
LOT No.   09182002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum((ntergen RB52405). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.1% trypsin. Subculture once a week and medium change twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:12
D7S820:10,13
D16S539:11
VWA:16
TH01:7,10
AM:X
TPOX:11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1022 Cell Name   OUMS-23
LOT No.   10232015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   approx. 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
Cell No.   JCRB1022 Cell Name   OUMS-23
LOT No.   02102020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.5 - 3.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   80 Additional information   
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