JCRB1028 JHH-2
Cell information
Important Notice(s)On the agreement for distribution of JHH series cell lines, NOZ and OZ
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1028 | Cell Name | JHH-2 |
|---|---|---|---|
| Profile | Human hepatocellular carcinoma cell line (Ed.II). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 57 |
| Identity | available | Tissue for Primary Cancer | liver, gallbladder |
| Case history | hepatocellular carcinoma. HBs-Ag(-), HBs-Ab(-), AFP=6,100ng/ml, CEA 4.6 ng/ml. | Metastasis | Yes |
| Tissue Metastasized | ascites | Genetics | Integration of the HBV-DNA was not observed. |
| Life Span | infinite | Crisis PDL | |
| Morphology | spindle shaped cells | Character | hepatoma (Ed.II). HBs-Ag(-). The cells can be transplantable to nude mice. Mode of chromosome is 70. |
| Classify | tumor | Established by | S.Honma & Nagamori,S. |
| Registered by | Nagamori,S. | Regulation for Distribution | Restricted: Cell's developer's approval is required |
| Comment | Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. | Year | 1988 |
| Medium | Williams'E medium with 10% fetal calf serum originally and later 2% FCS is used for 5 years. (alternative MEM 90% + FBS 10%) | Methods for Passages | Cells are treated with 0.02% EDTA and 0.25% trypsin |
| Cell Number on Passage | 1 x 10^5 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | liver |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1701409 | Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines. Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K Hepatogastroenterology. 1990 Oct;37(5):457-60 |
| Pubmed id:2484807 | [Protein secretion of human cultured liver cells]. Nagamori S,Fujise K,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H Hum Cell. 1988 Dec;1(4):382-90 |
| Research results by users. Click！ | |
|---|---|
| Pubmed id:22309595 |
Fibroblast growth factor 19 expression correlates with tumor progression and poorer prognosis of hepatocellular carcinoma. Miura S,Mitsuhashi N,Shimizu H,Kimura F,Yoshidome H,Otsuka M,Kato A,Shida T,Okamura D,Miyazaki M BMC Cancer. 2012 Feb 6;12():56 |
| Pubmed id:20044606 |
Infrequent amplification of JUN in hepatocellular carcinoma. Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T Anticancer Res. 2009 Dec;29(12):4989-94 |
| Pubmed id:17295779 |
Cimetidine inhibits epidermal growth factor-induced cell signaling. Fujikawa T,Shiraha H,Nakanishi Y,Takaoka N,Ueda N,Suzuki M,Shiratori Y J Gastroenterol Hepatol. 2007 Mar;22(3):436-43 |
| Pubmed id:9446792 |
Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1. Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21 |
| Pubmed id:8835345 |
Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines. Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M J Med Virol. 1996 Feb;48(2):133-40 |
| Pubmed id:1315167 |
Induction of allogeneic tumour- and lymphokine-activated lymphocytes against hepatocellular carcinoma. Yasumura S,Higuchi K,Hioki O,Okada K,Tsukishiro T,Tsuchida T,Miyagiwa M,Nambu S,Yasuyama T,Inoue K J Gastroenterol Hepatol. 1992 Mar-Apr;7(2):136-41 |
| Pubmed id:1701409 |
Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines. Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K Hepatogastroenterology. 1990 Oct;37(5):457-60 |
| Pubmed id:3005723 |
[Effects of hyperthermia on the proliferation and viability of cultured human hepatobiliary carcinoma cells]. Homma S,Nagamori S,Fujise K,Sujino H,Matsuura T,Simizu K,Kameda H Nihon Shokakibyo Gakkai Zasshi. 1985 Sep;82(9):2043-8 |
| Pubmed id:none |
THE MORPHOLOGICAL STUDY OF HUMAN CULTURED HEPATOMA CELLS BY A NEW PLASMA POLYMERIZATION REPLICA METHOD AND TEM Hajime SUJINO Jikeikai Medical Journal 33(4), 379-400 (1986) |
| Pubmed id:none |
樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム 藤瀬 清隆, 藤多 和信, 永森 静志, 蓮村 哲, 本間 定, 筋野 甫, 松浦 知和, 清水 恵一郎, 新谷 稔, 大野 典也, 亀田 治男 肝臓 29(5), 697-698 (1988) |
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LOT Information
| Cell No. | JCRB1028 | Cell Name | |
|---|---|---|---|
| LOT No. | 09072011 | Lot Specification | distribution |
| Medium | Williams' medium E with 2% fetal bovine serum (FBS: GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 1.0 - 1.5 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 33 hrs. | Cell Number in Vial (cells/1ml) | 8.2 x 10^5 |
| Viability at cell freezing (%) | 93.1 | Antibiotics Used | free |
| Passage Number | P25 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Williams' medium E. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1028 | Cell Name | |
|---|---|---|---|
| LOT No. | 11022010 | Lot Specification | distribution |
| Medium | Williams' medium E with 2% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 1 - 3 x 10^4 cells/ml | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 2 days. | Cell Number in Vial (cells/1ml) | 5.1 x 10^5 |
| Viability at cell freezing (%) | 83.6 | Antibiotics Used | fre |
| Passage Number | P25 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Williams' medium E | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1028 | Cell Name | |
|---|---|---|---|
| LOT No. | 01222008 | Lot Specification | distribution |
| Medium | Williams' E medium with 2% FBS. A higher serum concentration (10%) can be used for good growth. (FBS lot; GIBCO 484375) | Temperature | 37 C |
| Cell Density at Seeding | 1 - 2 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | 35 - 70 hrs | Cell Number in Vial (cells/1ml) | 6.0 x 10^5 |
| Viability at cell freezing (%) | 94.4 | Antibiotics Used | free |
| Passage Number | P25 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Williams' E medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1028 | Cell Name | JHH-2 |
|---|---|---|---|
| LOT No. | 02072003 | Lot Specification | distribution |
| Medium | Williams' E medium with 2% fetal calf serum (Intergen RB52305). | Temperature | 37 C |
| Cell Density at Seeding | 4.0 x 10^3 cells/sq.cm. | Methods for Passages | Cells harvested after treatment with 0.2% trypsin and 0.02% EDTA. Subculture once a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 75.6 | Antibiotics Used | free |
| Passage Number | P22 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(type B), NP, LDH examined. Human not HeLa. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1028 | Cell Name | JHH-2 |
|---|---|---|---|
| LOT No. | 05022018 | Lot Specification | distribution |
| Medium | Williams' medium E with 2% fetal bovine serum (FBS: Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 1.3 - 3.3 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 59 hrs. | Cell Number in Vial (cells/1ml) | 9.3 x 10^5 |
| Viability at cell freezing (%) | 90.0 | Antibiotics Used | free |
| Passage Number | P26 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Williams' medium E | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 90.0 | Additional information |