JCRB1042 BSL2KA
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB1042 | Cell Name | BSL2KA |
|---|---|---|---|
| Profile | EBV transformed B-cell lymphoblastoid cell line from human bloom syndrome patient. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Bloom's syndrome. | Metastasis | |
| Tissue Metastasized | Genetics | B-cell transformed with EBV. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | |
| Classify | transformed | Established by | Tachibana,A. |
| Registered by | Tachibana,A. | Regulation for Distribution | |
| Comment | Virus test requires final confirmation. | Year | 2002 |
| Medium | RPMI1640 medium with 15% fetal calf serum. | Methods for Passages | Dilution before cell density reached to 1x10^6 cells/ml. |
| Cell Number on Passage | 2-3 x 10^5 cells/ml | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | Peripheral blood |
| Tissue Type | lymphoblastoid |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1042 | Cell Name | BSL2KA |
|---|---|---|---|
| LOT No. | 06112004 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 15% fetal calf serum. | Temperature | 37C |
| Cell Density at Seeding | 1.5 x 10^5 cells / ml | Methods for Passages | Pippeting and dilute |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.89x10^6 |
| Viability at cell freezing (%) | 94.9 | Antibiotics Used | Free |
| Passage Number | P12* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | G6PD(typeB),NP,LDH examined. Human not HeLa. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | Nt |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
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